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DNA methylation changes from primary cultures through senescence-bypass in Syrian hamster fetal cells initially exposed to benzo[a]pyrene

ABSTRACT: DNA methylation changes from primary cultures through senescence-bypass in Syrian hamster fetal cells initially exposed to benzo[a]pyrene

PROVIDER: PRJNA909278 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11026960.fastq.gz	Fastqsanger.gz
	SRR11026961.fastq.gz	Fastqsanger.gz
	SRR11026962.fastq.gz	Fastqsanger.gz
	SRR11026963.fastq.gz	Fastqsanger.gz
	SRR11026964.fastq.gz	Fastqsanger.gz

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DNA methylation changes from primary cultures through senescence-bypass in Syrian hamster fetal cells initially exposed to benzo[a]pyrene

Project description:This SuperSeries is composed of the SubSeries listed below. Purpose: Historically, the Syrian Hamster Embryo - cell transformation assay (SHE-CTA) has provided a method to predict the carcinogenicity of test chemicals. Note that the cells harvested for the SHE-CTA are from fetuses at gestation day 13.5 and not from embryos as historically recorded. This method has been criticized for insufficient mechanistic understanding and subjective assessment of colonies morphological transformation. A more objective method is needed that additionally provides mode of action information. A possible mode of action of carcinogens includes disruption of DNA methylation in gene regulatory regions and consequent changes in gene expression. Such genetic loci could provide biomarkers to assist in predicting the carcinogenicity of both genotoxic and non-genotoxic chemicals acting through DNA methylation disruption.

2023-02-10 | GSE220238 | GEO

Chronology of DNA methylation changes through transformation stage leading to senescence by-pass in Syrian hamster fetal primary cells exposed to benzo[a]pyrene

Project description:Purpose: To use reduced representaion bisulfite sequencing to measure differential genome methylation among: 1. cells treated with benzo[a]pyrene (B[a]P) versus vehicle alone (DMSO) using the Syrian Hamster Cell Transformation Assay method; 2. cells originating from normal (Nc) versus morphologically transformed (MTc) colonies; 3. cells in senescence (SEN) versus those that have bypassed senescence (SENbp).

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DNA methylation in Syrian hamster female fetal cells (gestation day 13.5) exposed in vitro to 5-aza-2’deoxycytidine (5aCdR)

Project description:Purpose: To validate the reduced representation bisulfite sequencing (RRBS) methodology by comparing DNA methylation pattern in Syrian hamster fetal (SHF) primary cells to a SHF cell line (MT2) exposed to the DNA methyl transferase inhibitor,5aCdR. The MT2 cell line was originally derived from the expansion of a benzo[a]pyrene-induced morphologically transformed clone (Pickles et al 2016. Mutat.Res.Genet.Toxicol.Environ Mutagen. 802:50-58).

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