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Regulatory changes associated with the head to trunk developmental transition [ATAC-seq]

ABSTRACT: Regulatory changes associated with the head to trunk developmental transition [ATAC-seq]

PROVIDER: PRJNA909288 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22550003_1.fastq.gz	Fastqsanger.gz
	SRR22550003_2.fastq.gz	Fastqsanger.gz
	SRR22550004_1.fastq.gz	Fastqsanger.gz
	SRR22550004_2.fastq.gz	Fastqsanger.gz
	SRR22550005_1.fastq.gz	Fastqsanger.gz

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Project description:BackgroundDevelopment of vertebrate embryos is characterized by early formation of the anterior tissues followed by the sequential extension of the axis at their posterior end to build the trunk and tail structures, first by the activity of the primitive streak and then of the tail bud. Embryological, molecular and genetic data indicate that head and trunk development are significantly different, suggesting that the transition into the trunk formation stage involves major changes in regulatory gene networks.ResultsWe explored those regulatory changes by generating differential interaction networks and chromatin accessibility profiles from the posterior epiblast region of mouse embryos at embryonic day (E)7.5 and E8.5. We observed changes in various cell processes, including several signaling pathways, ubiquitination machinery, ion dynamics and metabolic processes involving lipids that could contribute to the functional switch in the progenitor region of the embryo. We further explored the functional impact of changes observed in Wnt signaling associated processes, revealing a switch in the functional relevance of Wnt molecule palmitoleoylation, essential during gastrulation but becoming differentially required for the control of axial extension and progenitor differentiation processes during trunk formation. We also found substantial changes in chromatin accessibility at the two developmental stages, mostly mapping to intergenic regions and presenting differential footprinting profiles to several key transcription factors, indicating a significant switch in the regulatory elements controlling head or trunk development. Those chromatin changes are largely independent of retinoic acid, despite the key role of this factor in the transition to trunk development. We also tested the functional relevance of potential enhancers identified in the accessibility assays that reproduced the expression profiles of genes involved in the transition. Deletion of these regions by genome editing had limited effect on the expression of those genes, suggesting the existence of redundant enhancers that guarantee robust expression patterns.ConclusionsThis work provides a global view of the regulatory changes controlling the switch into the axial extension phase of vertebrate embryonic development. It also revealed mechanisms by which the cellular context influences the activity of regulatory factors, channeling them to implement one of several possible biological outputs.

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Regulatory changes associated with the head to trunk developmental transition [ATAC-seq]

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