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The regulons of an OmpR-like transcription factor in Pseudomonas syringae pv. Actinidiae

ABSTRACT: The regulons of an OmpR-like transcription factor in Pseudomonas syringae pv. Actinidiae

PROVIDER: PRJNA909400 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22557401_1.fastq.gz	Fastqsanger.gz
	SRR22557401_2.fastq.gz	Fastqsanger.gz
	SRR22557402_1.fastq.gz	Fastqsanger.gz
	SRR22557402_2.fastq.gz	Fastqsanger.gz

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The regulons of an OmpR-like transcription factor in Pseudomonas syringae pv. Actinidiae

Project description:A ChIP-seq assay was performed to identify the regulons of an ompR-like transcription factor (gene name: 13375, GenBank: AYL80818.1) in Pseudomonas syringae pv. actinidiae. An 13375-overexpressing mutant G1-OE13375, which constitutively express C-terminally Myc-tagged ompR-like gene in the 13375-deletion mutant G1Δ13375, was used in this study. The bacterial cells were cultured either in nutrition-rich KB medium or hrp-derepressing medium (HDM) at 25 C for 24 hours. A PierceTM Magnetic ChIP Kit (Cat. #: 26157, Thermo Fisher Scientific) and a ChIP-grade Myc-Tag Monoclonal Antibody (Myc.A7, Cat. #: MA121316, Thermo Fisher Scientific) were used for sample pretreatment and immunoprecipitation.

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