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Characterization of a human regulatory T cell-like cell line, MT-2, in the presence or absence of FOXP3

ABSTRACT: Characterization of a human regulatory T cell-like cell line, MT-2, in the presence or absence of FOXP3

PROVIDER: PRJNA910568 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22600584_1.fastq.gz	Fastqsanger.gz
	SRR22600584_2.fastq.gz	Fastqsanger.gz
	SRR22600585_1.fastq.gz	Fastqsanger.gz
	SRR22600585_2.fastq.gz	Fastqsanger.gz
	SRR22600586_1.fastq.gz	Fastqsanger.gz

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Characterization of a human regulatory T cell-like cell line, MT-2, in the presence or absence of FOXP3

Project description:These studies profiled the transcriptional differences in a human regulatory T cell-like cell line, MT-2, when FOXP3 a master transcriptional regulatory element was present or absence. In the FOXP3 null MT-2 cell line FOXP3 express had been deleted by CRISPR-Cas9. RNA was prepared for bulk RNA-seq from 4 replicates for each cell line. In the absence of FOXP3 in the MT-2 cell line there were 1251 differential expressed genes. These differentially expressed gene results indicate that hte loss of FOXP3 in MT-2 cells results in altered transcripts and pathways often associated with regultory T cell activity or phenotypes.

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Transcriptional Analysis of Type II Alveolar Epithelial Cells in the Presence or Absence of Foxp3+ Regulatory T Cells during Experimental Acute Lung Injury Resolution

Project description:These studies profiled the expression of mRNA in lung type II alveolar epithelial cells during lipopolysacchride induced lung injury in the presence or absence of Foxp3+ Regulatory T Cells Baseline, uninjured lung type II alveolar epithelial (AT2) cells or resolving AT2 cells, in the presence or absence of Foxp3+ regulatory T cells (Tregs), were sorted (7 days after intratracheal instillation of LPS for resolving conditions) and mRNAs differentially expressed (DE) between control AT2 cells, resolving AT2 cells with Tregs present, or resolving AT2 cells in mice depleted of Tregs were identified using microarrays.

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Transcription profiling of mouse CD4+ T cells expressing Foxp3 FACS isolated from hemizygouse males to investigate development of regulatory t cell precursors in the absence of a functional foxp3 protein

Project description:To analyze gene expression in in regulatory T cell precursors that develop in the absence of a functional Foxp3 protein as compared to that of normal regulatory T cells Experiment Overall Design: Murine wild-type and mutant (delta) Foxp3 alleles were tagged with EGFP reporters. CD4 positive T cells expressing the respective tagged Foxp3 allele (Foxp3/EGFP and delta Foxp3/EGFP) were isolated from hemizygous male mice by cell sorting using FACS. CD4 positive cells that did not express the EGFP tag were a isolated. Total RNA was prepared and used in the array studies.

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