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Ribosomal frameshifting at normal codon repeats recodes functional chimeric proteins in human[ChIP-seq]

ABSTRACT: Ribosomal frameshifting at normal codon repeats recodes functional chimeric proteins in human[ChIP-seq]

PROVIDER: PRJNA910965 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22677773.fastq.gz	Fastqsanger.gz
	SRR22677774.fastq.gz	Fastqsanger.gz
	SRR22677775.fastq.gz	Fastqsanger.gz

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Ribosomal frameshifting at normal codon repeats recodes functional chimeric proteins in human [RNA-seq]

Project description:Ribosomal frameshifting refers to the process that ribosomes slip into +1 or -1 reading frame, thus produce chimeric trans-frame proteins. In viruses and bacteria, programmed ribosomal frameshifting can produce essential trans-frame proteins for viral replication or regulation of other biological processes. In humans, however, functional trans-frame protein derived from ribosomal frameshifting is scarcely documented. Combining multiple assays, we show that short codon repeats could act as cis-acting elements that stimulate ribosomal frameshifting in humans, abbreviated as CRFS hereafter. Using proteomic analyses, we identified many putative CRFS events from 32 normal human tissues supported by trans-frame peptides positioned at codon repeats. Finally, we show a CRFS-derived trans-frame protein (HDAC1-FS) functions by antagonizing the activities of HDAC1, thus affecting cell migration and apoptosis. These data suggest a novel type of translational recoding associated with codon repeats, which may expand the coding capacity of mRNA and diversify the regulation in human.

2023-05-01 | GSE220677 | GEO

Ribosomal frameshifting at normal codon repeats recodes functional chimeric proteins in human[ChIP-seq]

Project description:Ribosomal frameshifting refers to the process that ribosomes slip into +1 or -1 reading frame, thus produce chimeric trans-frame proteins. In viruses and bacteria, programmed ribosomal frameshifting can produce essential trans-frame proteins for viral replication or regulation of other biological processes. In humans, however, functional trans-frame protein derived from ribosomal frameshifting is scarcely documented. Combining multiple assays, we show that short codon repeats could act as cis-acting elements that stimulate ribosomal frameshifting in humans, abbreviated as CRFS hereafter. Using proteomic analyses, we identified many putative CRFS events from 32 normal human tissues supported by trans-frame peptides positioned at codon repeats. Finally, we show a CRFS-derived trans-frame protein (HDAC1-FS) functions by antagonizing the activities of HDAC1, thus affecting cell migration and apoptosis. These data suggest a novel type of translational recoding associated with codon repeats,which may expand the coding capacity of mRNA and diversify the regulation in human.

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Ribosomal frameshifting at normal codon repeats recodes functional chimeric proteins in human.

Project description:Ribosomal frameshifting at normal codon repeats recodes functional chimeric proteins in human.

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Ribosomal frameshifting at normal codon repeats recodes functional chimeric proteins in human [RNA-seq]

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Start codon-associated ribosomal frameshifting mediates nutrient stress adaptation

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Ribosomal frameshifting at normal codon repeats recodes functional chimeric proteins in human.

Project description:This SuperSeries is composed of the SubSeries listed below.

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