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ABSTRACT: Acetic acid tolerance in probiotic yeast Saccharomyces var. boulardii.

PROVIDER: PRJNA911196 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22682660_1.fastq.gz	Fastqsanger.gz
	SRR22682660_2.fastq.gz	Fastqsanger.gz
	SRR22805713_1.fastq.gz	Fastqsanger.gz
	SRR22805713_2.fastq.gz	Fastqsanger.gz
	SRR22824388.fastq.gz	Fastqsanger.gz

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Comparison of S. boulardii cells in H2O vs. S. boulardii cells in H2O+HCl

Project description:We sought to better understand the mechanism of acid-induced cell death in Saccharomyces boulardii--a probiotic yeast routinely used to prevent and treat gastrointestinal disorders. To do this we generated microarray gene expression profiles of S. boulardii cells cultured in an acidic environment.

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Comparison of S. boulardii cells in H2O vs. S. boulardii cells in H2O+HCl

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Comparative genomic hybridization of Saccharomyces boulardii and Saccharomyces cerevisiae to determine genomic differences

Project description:A comparative genomic hybridisation experiment using Affymetrix YG-S98 arrays to study the genetic background of S. Boulardii compared to S. Cerevisiae strain BY4743. Background: Saccharomyces boulardii, a yeast that was isolated from fruit in Indochina has been used as a remedy for diarrhoea since 1950, and is now a commercially available treatment throughout Europe, Africa and South America. Though initially classified as a separate species of Saccharomyces, recent publications have shown that the genome of S. boulardii is so similar to Saccharomyces cerevisiae that the two should be classified as conspecific. This raises the question of the distinguishing molecular and phenotypic characteristics present in S. boulardii that make it perform more effectively as a probiotic organism compared to other strains of S. cerevisiae. This investigation reports some of these characteristics including enhanced ability for pseudohyphal switching upon nitrogen limitation and increased resistance to acidic pH. However, these differences did not correlate with increased adherence to epithelial cells or transit through mouse gut. Pertinent characteristics of the S. boulardii genome such as trisomy of chromosome IX, altered copy number of a number of individual genes and sporulation deficiency have been revealed by comparative genome hybridisation using oligonucleotide-based microarrays coupled with a rigorous statistical analysis. The contributions of the different genomic and phenotypic features of S. boulardii to its probiotic nature are discussed.

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Saccharomyces cerevisiae strain:PY0001

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Project description:Extracellular vesicles (EVs) are nanoparticles containing various bioactive cargos, e.g., proteins, RNAs, and lipids, that are released into the environment by all cell types. They are involved in, amongst others, intercellular communication. This article presents studies on EVs produced by the probiotic yeast Saccharomyces boulardii CNCM I-745. The size distribution and concentration of EVs in the liquid culture of yeast were estimated. Moreover, the vesicles of S. boulardii were tested for their cytotoxicity against three model human intestinal cell lines. This study did not show any significant negative effect of yeast EVs on these cells under tested conditions. In addition, EVs of S. boulardii were verified for their ability to internalize in vitro with human cells and transfer their cargo. The yeast vesicles were loaded with doxorubicin, an anticancer agent, and added to the cellular cultures. Subsequently, microscopic observations revealed that these EVs transferred the chemotherapeutic to human intestinal cell lines. A cytotoxicity test confirmed the activity of the transferred doxorubicin. Detailed information about the proteins present in EVs might be important in terms of exploring yeast EVs as carriers of active molecules. Thus, proteomic analysis of the EV content was also conducted within the present study, and it allowed to identify 541 proteins after matching to the Saccharomyces Genome Database (SGD). Altogether, this study provides strong evidence that the EVs of the probiotic CNCM I-745 strain could be considered a drug delivery system.

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