Project description:Here we explore the striatum transcriptomic effects of alpha-synuclein administration in mice as model of Parkinson´s disease. Moreover, we also explore the neuroprotective effect of cannabonoid-based druga (VCE-003.2) as a potential neuroportective agent in Parkinson´s disease
Project description:High throughput sequencing of small RNAs (mature miRNA and piRNA/piRNA-like molecules) from control- and sporadic Parkinson´s disease patient derived fibroblasts (n=15), induced pluripotent stem cells (n=26) and differentiated midbrain neurons (n=10). In parallel, cingulate gyrus samples from eight control- and eight Parkinson´s disease patients obtained from the Netherlands Brain Bank were sequenced. We found significant differences between the groups in every cell type and in tissue.
Project description:High throughput sequencing of coding exome RNA from cingylate gyrus brain tissue obtained from control- and Parkinson´s disease patients. In the Parkinson´s disease affected tissue, changes are present that are known to be related to the pathogenesis of neurodegenerative diseases.
Project description:Peripheral blood samples were collected from (Heavy metal exposer- Parkinson disease)HME-PD, (Heavy metal non-exposer- Parkinson disease) NHME-PD, and healthy controls. Total RNA was isolated and profiled using the Affymetrix GeneChip™ miRNA 3.1 Array. Arrays were hybridized, washed, stained, scanned, and raw data (.CEL files) were RMA-normalized for differential expression.
Project description:High throughput sequencing of poly-A RNA from control- and sporadic Parkinson´s disease patient derived fibroblasts (n=15), induced pluripotent stem cells (n=31) and differentiated midbrain neurons (n=15). Fibroblasts and iPSCs do not show major differences on single gene level. In contrast, midbrain neurons derived from Parkinson´s disease patients show changes known to be associated with neurodegenerative diseases.
