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Serpine1 functions as a non-coding RNA regulating post-transcriptionally epithelial mesenchymal transition

ABSTRACT: Serpine1 functions as a non-coding RNA regulating post-transcriptionally epithelial mesenchymal transition

PROVIDER: PRJNA913119 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22752450_1.fastq.gz	Fastqsanger.gz
	SRR22752450_2.fastq.gz	Fastqsanger.gz
	SRR22752451_1.fastq.gz	Fastqsanger.gz
	SRR22752451_2.fastq.gz	Fastqsanger.gz
	SRR22752452_1.fastq.gz	Fastqsanger.gz

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Serpine1 functions as a non-coding RNA regulating post-transcriptionally epithelial mesenchymal transition [iCLIP]

Project description:Serpine1 functions as a non-coding RNA regulating post-transcriptionally epithelial mesenchymal transition [iCLIP]

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Serpine1 functions as a non-coding RNA regulating post-transcriptionally epithelial mesenchymal transition [iCLIP]

Project description:Serpine1 mRNA is abundantly induced during EMT. Paradoxically, this mRNA is enriched in the RISC complex, blocking its translation and acting as an endogenous RNA competitor for several miRNAs. Cells that overexpress Serpine1 mRNA have a greater capacity for migration, invasiveness, and resistance to anoikis. These cells show an altered proteomic profile, highlighting the increased levels of expression of the splicing factor TRA2b. This splicing factor is posttranscriptionally regulated during EMT by upregulation of Serpine1 mRNA and the resulting sequestration of miR-130b-5p by this mRNA, which contains three binding sites for miR-130b-5p. Through transcriptomic analysis, we confirmed that Serpin 1 mRNA expression modulates the expression of numerous genes, most of which (>60%) are also modulated by TRA2b overexpression.

2023-12-15 | GSE221152 | GEO

Serpine1 functions as a non-coding RNA regulating post-transcriptionally epithelial mesenchymal transition [RNA-seq]

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Serpine1 functions as a non-coding RNA regulating post-transcriptionally epithelial mesenchymal transition [RNA-seq]

Project description:Serpine1 functions as a non-coding RNA regulating post-transcriptionally epithelial mesenchymal transition [RNA-seq]

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Serpine1 functions as a non-coding RNA regulating post-transcriptionally epithelial mesenchymal transition

Project description:This SuperSeries is composed of the SubSeries listed below.

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Diverse roles of SERPINE1 in regulating cellular proliferation and invasion

Project description:SERPINE1 is involved in various biological processes, but its roles in promoting or suppressing tumorigenesis remain controversial. To understand the underlying mechanisms, we focused on the effects of SERPINE1 downregulation on cell phenotypes, particularly proliferation and invasion, across three types of tumors. High SERPINE1 levels in breast cancer (BRCA) and low-grade glioma (LGG) were associated with poor prognosis. In contrast, elevated SERPINE1 levels in skin cutaneous melanoma (SKCM) correlated with better outcomes. SERPINE1 knockdown resulted in increased xenograft growth in the melanoma cell line C918. This was characterized by the promotion of the cell cycle through the modulation of minichromosome maintenance protein expression and the activity of p53 and SMAD3. In breast cancer cells (MDA-MB-231) with SERPINE1 knockdown, there was decreased xenograft growth and cell proliferation, attributed to a reduction in the uPAR-mediated ERK/p38 activity ratio. With SERPINE1 knockdown, both C918 and MDA-MB-231 cells demonstrated reduced invasion capabilities, decreased matrix metalloproteinase (MMP) activity, and reduced lung metastasis. In low-grade glioma cells (H4), SERPINE1 knockdown led to decreased cell proliferation due to a reduction in the HSP90-mediated ERK/p38 activity ratio. However, it increased invasion and MMP activity, particularly of MMP-1, regulated by the HSP90-p38 axis. Collectively, our findings reveal that SERPINE1 exerts diverse effects on cell proliferation and invasion through context-dependent mechanisms. These results suggest that targeting SERPINE1 may offer personalized therapeutic strategies to enhance treatment precision and reduce adverse effects.

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EPB41L5 functions to post-transcriptionally regulate cadherin and integrin during epithelial-mesenchymal transition.

Project description:EPB41L5 belongs to the band 4.1 superfamily. We investigate here the involvement of EPB41L5 in epithelial-mesenchymal transition (EMT) during mouse gastrulation. EPB41L5 expression is induced during TGFbeta-stimulated EMT, whereas silencing of EPB41L5 by siRNA inhibits this transition. In EPB41L5 mutants, cell-cell adhesion is enhanced, and EMT is greatly impaired during gastrulation. Moreover, cell attachment, spreading, and mobility are greatly reduced by EPB41L5 deficiency. Gene transcription regulation during EMT occurs normally at the mRNA level; EPB41L5 siRNA does not affect either the decrease in E-cadherin or the increase in integrin expression. However, at the protein level, the decrease in E-cadherin and increase in integrin are inhibited in both EPB41L5 siRNA-treated NMuMG cells and mutant mesoderm. We find that EPB41L5 binds p120ctn through its N-terminal FERM domain, inhibiting p120ctn-E-cadherin binding. EPB41L5 overexpression causes E-cadherin relocalization into Rab5-positive vesicles in epithelial cells. At the same time, EPB41L5 binds to paxillin through its C terminus, enhancing integrin/paxillin association, thereby stimulating focal adhesion formation.

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Project description:To determine the function of Serpine1 in alveolar cells, the Serpine1 overexpression plasmid pCI-neo-Serpine1 was constructed and transfected into MLE-12 cells

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CDCA7 promotes TGF-β-induced epithelial-mesenchymal transition via transcriptionally regulating Smad4/Smad7 in ESCC

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SM004 - SARS infection of C57BL6, TIMP1 and Serpine1 knock-out mice.

Project description:This purpose of this experiment was to investigate the transcriptional differences between C57BL6, TIMP1 and Serpine1 knock-out mice infected with SARS MA15 virus. Overview of Experiment: Groups of 20 week old C57BL6, TIMP1 and Serpine1 (PAI1) knock-out mice were infected with SARS MA15 virus. Infections were done at 10^4 PFU or time-matched mock infected. Time points were 4 and 7 d.p.i. There were 2-4 animals/dose/time point. Lung samples were collected for virus load, transcriptional and proteomics analysis. Weight loss and animal survival were also monitored.

2014-02-20 | E-GEOD-51386 | biostudies-arrayexpress

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