Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Maintenance of pluripotency in the entire ectoderm enables neural crest formation and challenges dogmatic viewpoints of gastrulation

ABSTRACT: Maintenance of pluripotency in the entire ectoderm enables neural crest formation and challenges dogmatic viewpoints of gastrulation

PROVIDER: PRJNA913169 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR22779145.fastq.gz	Fastqsanger.gz
	SRR22779146.fastq.gz	Fastqsanger.gz
	SRR22779147.fastq.gz	Fastqsanger.gz
	SRR22779148.fastq.gz	Fastqsanger.gz
	SRR22779149.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 16

Similar Datasets

Maintenance of pluripotency in the entire ectoderm enables neural crest formation and challenges dogmatic viewpoints of gastrulation

Project description:Maintenance of pluripotency in the entire ectoderm enables neural crest formation and challenges dogmatic viewpoints of gastrulation

| PRJNA913168 | ENA

Maintenance of pluripotency in the entire ectoderm enables neural crest formation and challenges dogmatic viewpoints of gastrulation

Project description:The ability of the pluripotent epiblast to contribute progeny to all three germ layers is thought to be lost after gastrulation. The later-forming neural crest (NC) rises from ectoderm and it remains poorly understood how its exceptionally high stem-cell potential to generate mesodermal- and endodermal-like cells is obtained. We monitored transcriptional changes from gastrulation to neurulation using single-cell-Multiplex-Spatial-Transcriptomics (scMST) complemented with RNA-sequencing. Unexpectedly, we find maintenance of undecided Nanog/Oct4-PouV/Klf4-positive pluripotent-like pan-ectodermal stem-cells spanning the entire ectoderm late in the neurulation process with ectodermal patterning completed only at the end of neurulation when pluripotency becomes restricted to NC, challenging our understanding of gastrulation. Furthermore, broad ectodermal pluripotency is found at all axial levels unrelated to the NC lineage the cells later commit to, suggesting a general role in stemness enhancement and proposing a mechanism by which the NC acquires its ability to form derivatives beyond “ectodermal-capacity” in chick and mouse embryos.

2023-03-02 | GSE221188 | GEO

Maintenance of pluripotency in the entire ectoderm enables neural crest formation and challenges dogmatic viewpoints of gastrulation

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-03-02 | GSE221190 | GEO

Maintenance of pluripotency signature in the entire ectoderm enables potential for neural crest formation

2023-08-28 | GSE232432 | GEO

Maintenance of pluripotency signature in the entire ectoderm enables potential for neural crest formation [bulk_RNASeq]

Project description:The ability of the pluripotent epiblast to contribute progeny to all three germ layers is thought to be lost after gastrulation. The later-forming neural crest (NC) rises from ectoderm and it remains poorly understood how its exceptionally high stem-cell potential to generate mesodermal- and endodermal-like cells is obtained. We monitored transcriptional changes from gastrulation to neurulation using single-cell-Multiplex-Spatial-Transcriptomics (scMST) complemented with RNA-sequencing. Here we show maintenance of pluripotency-signature (Nanog/Oct4-PouV/Klf4-positive) in undecided pan-ectodermal stem-cells spanning the entire ectoderm late during neurulation with ectodermal patterning completed only at the end of neurulation when the pluripotency-signature becomes restricted to NC, challenging our understanding of gastrulation. Furthermore, broad ectodermal pluripotency-signature is found at all axial levels unrelated to the NC lineage the cells later commit to, suggesting a general role in stemness enhancement and proposing a mechanism by which the NC acquires its ability to form derivatives beyond “ectodermal-capacity” in chick and mouse embryos.

2023-03-02 | GSE221125 | GEO

BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation

Project description:Cell migration is a fundamental process during embryonic development. Most studies in vivo have focussed on the migration of cells using the extracellular matrix (ECM) as their substrate for migration. In contrast, much less is known about how cells migrate on other cells, as found in early embryos when the ECM has not yet formed. Here, we show that lateral mesendoderm (LME) cells in the early zebrafish gastrula use the ectoderm as their substrate for migration. We show that the lateral ectoderm is permissive for the animal pole-directed migration of LME cells, while the ectoderm at the animal pole halts it. These differences in the permissiveness depend on the lateral ectoderm being more cohesive than the animal ectoderm, a property controlled by BMP signalling within the ectoderm. Collectively, these findings identify ectoderm tissue cohesion as one critical factor regulating LME migration during zebrafish gastrulation.

2025-01-07 | GSE251904 | GEO

UMI-4C in wild-type and p63-null surface ectoderm cells

Project description:Purpose: Validate HiChIP-identified long-range chromatin interactions at the TFAP2C locus, in a viewpoint-specific manner. Methods: UMI-4C libraries targeting 3 individual viewpoints at the TFAP2C locus were generated in duplicate, in surface ectoderm cells with and without p63. Replicates were sequenced on Illumina NextSeq sequencer, and reads were aligned and filtered for valid long-range interactions using HiCPro. Results: Dynamic long-range interactions at the TFAP2C locus were detected with and without p63 in surface ectoderm cells. Conclusion: Long-range interactions detected via HiChIP were confirmed in the surface ectoderm by this technique, validating the HiChIP method through an orthogonal approach.

2018-11-06 | GSE114846 | GEO

Maintenance of pluripotency signature in the entire ectoderm enables potential for neural crest formation

Project description:Maintenance of pluripotency signature in the entire ectoderm enables potential for neural crest formation

| PRJNA971972 | ENA

Maintenance of pluripotency signature in the entire ectoderm enables potential for neural crest formation [bulk_RNASeq]

Project description:Maintenance of pluripotency signature in the entire ectoderm enables potential for neural crest formation [bulk_RNASeq]

| PRJNA913002 | ENA

Single cell chromatin accessibility profiles of the chick embryonic ectoderm

Project description:The embryonic ectoderm gives rise to four fates: neural, neural crest, placodal and epidermal. The derivatives of these fates give rise to the entire vertebrate nervous system and skin. To explore how these cell fates emerge, we profiled single cell chromatin accessibility in the chick embryonic ectoderm at five developmental timepoints.

2025-12-02 | GSE271424 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data