Ontology highlight
ABSTRACT:
PROVIDER: PRJNA914723 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR22862544_1.fastq.gz | Fastqsanger.gz | |||
| SRR22862544_2.fastq.gz | Fastqsanger.gz | |||
| SRR22862545_1.fastq.gz | Fastqsanger.gz | |||
| SRR22862545_2.fastq.gz | Fastqsanger.gz | |||
| SRR22862546_1.fastq.gz | Fastqsanger.gz |
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Similar Datasets
Project description:The biting behavior observed in Carpenter ants infected by the specialized fungus Ophiocordyceps unilateralis s.l. is an example of a complex host behavioral manipulation by parasite. Though parasitic manipulation of host behavior is generally assumed to be due to the parasite’s gene expression, few studies have set out to test this. We experimentally infected Carpenter ants to collect tissue from both parasite and host during the time period when manipulated biting behavior is experienced. Upon observation of synchronized biting, samples were collected and subjected to RNA-Seq analyses. We also sequenced and annotated the O. unilateralis s.l. genome as a reference for the fungal reads. Our mixed transcriptomics approach, together with a comparative genomics study, shows that the majority of the fungal genes that are up-regulated during manipulated biting behavior are unique to the O. unilateralis s.l. genome. This study furthermore reveals that the fungal parasite might be regulating immune- and neuronal stress responses in the host during manipulated biting, as well as impairing its chemosensory communication and causing apoptosis. Moreover, we found genes up-regulated during manipulation that putatively encode for proteins with reported effects on behavioral outputs, proteins involved in various neuropathologies, and proteins involved in the biosynthesis of secondary metabolites such as alkaloids.
2015-08-18 | GSE68176 | GEO
Project description:The natural histories of various parasite-host interactions that involve adaptive manipulation of host behavior describe a time-of-day synchronization of certain events. One example is the manipulated biting behavior observed in Carpenter ants infected with Ophiocordyceps unilateralis sensu lato. Field observations in Thailand demonstrated that manipulated biting takes place around solar noon. Laboratory work with species from South Carolina, demonstrated a shift of this synchronized biting event to the early morning. We therefore hypothesize that biological clocks play an important role in these and other parasite-host interactions. To set the stage for our future research into this, we, here, aimed to explore if fungal species of the complex O. unilateralis s.l. have a functional molecular clock. We, therefore, performed RNA-Seq on time course samples of the recently sequenced species Ophiocordyceps kimflemingiae. Fungal blastospores grown in liquid media were harvested under cycling light-dark and continuous darkness conditions. Rhythmic mRNA levels were assessed with JTK_CYCLE. We found that O. kimflemingiae is not hugely rhythmic under our sampling conditions, but rhythmicity can be observed. Moreover, we identified functional homologs of known clock genes. In addition, our data indicated that regulation of transcription appears to take place during the light phase (day time). In contrast, the expression levels of a significant number of secreted enzymes, proteases, toxins and small bioactive compounds peaked during the dark phase (night time). This suggests that this fungal parasite might indeed rely on biological clocks for the precise daily timing of certain events and additionally validates further research into this.
2017-10-21 | GSE101312 | GEO
Project description:Gene expression during biting behavior in zombie ants reflects the complexity of fungal pathogenic manipulation of ants
| PRJNA281955 | ENA
Project description:<p>One of the main challenges of the socially parasitic mode of life is bypassing the host's recognition ability, which ensures that altruistic behaviour is directed towards related individuals. Various chemical strategies have evolved to achieve this goal. The most widespread, used also by the obligate slave-making ants, is camouflage or mimicry of colony odour encoded in cuticular hydrocarbon (CHC) composition. However, recent studies have shown that facultative slave-makers employ a different strategy: they manipulate the slaves' recognition labels to make them resemble the parasite's CHC profile. We examined the limitations of this strategy by focusing on incipient F. sanguinea colonies, where slaves are the majority. Our study revealed that callow F. sanguinea ants initially suppress their species-specific odour profile, which develops gradually over time accompanied by an increase of CHC amount per surface area in slave-maker workers. This allows the slaves to familiarise themselves with the parasite's CHC. We found that callow ants produce lower amounts of CHC, and the relative abundance of certain compounds differs from what is observed in older ants. Additionally, preimaginal stages of F. sanguinea ants acquire CHC from the slaves, which are later incorporated into the imagines’ recognition labels. These findings support the proposition that the parasite's manipulation strategy is limited by the slaves' learning capacity, which is necessary to maintain colony cohesion. They also shed light on the selective pressures that might have led to the evolution of chemical mimicry in mature obligate slave-maker colonies.</p>
2026-05-19 | MTBLS13712 | MetaboLights
Project description:This SuperSeries is composed of the following subset Series: GSE31344: smRNA sequencing of queen and virgin queen of two ants: Camponotus floridanus and Harpegnathos saltator GSE31346: Transcriptome sequencing of queen and virgin queen of two ants: Camponotus floridanus and Harpegnathos saltator GSE31576: Single base resolution methylome of two ants: Camponotus floridanus and Harpegnathos saltator Refer to individual Series
2012-08-08 | E-GEOD-31577 | biostudies-arrayexpress
Project description:Parasites with complex life cycles often manipulate the phenotype of their intermediate hosts to increase the probability of transmission to their definitive hosts. Infection with Anomotaenia brevis, a cestode that uses Temnothorax nylanderi ants as intermediate hosts, leads to a multiple-fold extension of host lifespan and to changes in behaviour, morphology and colouration. The mechanisms behind these changes are unknown, as is whether the increased longevity is achieved through parasite manipulation. Here, we demonstrate that the parasite releases proteins into its host with functions that might explain the observed changes. These parasitic proteins make up a substantial portion of the proteome of the hosts' haemolymph, and thioredoxin peroxidase and superoxide dismutase, two antioxidants, exhibited the highest abundances among them. The largest part of the secreted proteins could not be annotated, indicating they are either novel or severely altered during recent coevolution to function in host manipulation. We also detected shifts in the hosts' proteome with infection, in particular an overabundance of vitellogenin-like A in infected ants, a protein that regulates division of labour in Temnothorax ants, which could explain the observed behavioural changes. Our results thus suggest two different strategies that might be employed by this parasite to manipulate its host: secreting proteins with immediate influence on the host's phenotype and altering the host's translational activity. Our findings highlight the intricate molecular interplay required to influence the phenotype of a host and point to potential signalling pathways and genes involved in parasite-host communication.
2025-08-07 | PXD040025 | Pride
Project description:A two-speed genome architecture underlies the evolution of host manipulation in zombie-ant fungi
| PRJNA1453744 | ENA
Project description:Transcriptome of the entomopathogenic fungus, Entomophthora muscae, during behavioural manipulation of ‘zombie’-flies (Musca domestica)
| PRJEB45005 | ENA
Project description:The goal of this study was to assay the extent of variation in chromatin organization between 3 ant castes (major and minor female workers and males) in one colony of Camponotus floridanus carpenter ant using ChIPseq. 45 samples total: 30 ChIP samples and 3 inputs for total histone H3, 7 histone H3 PTMs and RNA Pol II in major, minor, and male ants; CBP in major and minor ants; the major H3K27ac sample was replicated. 4 ChIP samples for H3 and H3K27ac in brains of majors and minors, and 2 inputs. 2 RNAseq samples for major and minor ants head+thorax; 4 RNAseq samples for brain (majors and minors with 2 replicates each).
2012-11-26 | E-GEOD-37523 | biostudies-arrayexpress
Project description:We report transcriptomes of pre-sorted skin wound dermal cells. Post-wounding day (PWD) 12, 15 and 21 Zombie-neg;tdTomatoHi cells were FACS sorted from Sm22-Cre;TdTomato mice.
2018-12-05 | GSE113605 | GEO