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Single-cell atlas of human leptomeningeal immune cells

ABSTRACT: Single-cell atlas of human leptomeningeal immune cells

PROVIDER: PRJNA914729 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22882200_1.fastq.gz	Fastqsanger.gz
	SRR22882200_2.fastq.gz	Fastqsanger.gz
	SRR22882201_1.fastq.gz	Fastqsanger.gz
	SRR22882201_2.fastq.gz	Fastqsanger.gz
	SRR22882202_1.fastq.gz	Fastqsanger.gz

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Project description:Metastasis to the cerebrospinal fluid (CSF)-filled leptomeninges, or leptomeningeal metastasis (LM), represents a fatal complication of cancer. Proteomic and transcriptomic analyses of human CSF reveal a substantial inflammatory infiltrate in LM. We find the solute and immune composition of CSF in the setting of LM changes dramatically, with notable enrichment in IFN-gamma signaling. To investigate the mechanistic relationships between immune cell signaling and cancer cells within the leptomeninges, we developed syngeneic lung, breast, and melanoma LM mouse models. We find that transgenic host mice, lacking IFN-gamma or its receptor, fail to control LM growth. Overexpression of Ifng through a targeted AAV system controls cancer cell growth independent of adaptive immunity. Instead, leptomeningeal IFN-gamma actively recruits and activates peripheral myeloid cells, generating a diverse spectrum of dendritic cell subsets. These migratory, CCR7+ dendritic cells orchestrate the influx, proliferation, and cytotoxic action of natural killer cells to control cancer cell growth in the leptomeninges. This work uncovers leptomeningeal-specific IFN-gamma signaling and suggests a novel immune-therapeutic approach against tumors within this space.

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