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Lacticaseibacillus paracasei subsp. paracasei strain:GMNL-143

ABSTRACT: Exploiting next-generation sequencing and third-generation sequencing to assemble whole-genome sequences and annotate genes for functional probiotics of Lacticaseibacillus paracasei strain GMNL-143

PROVIDER: PRJNA915772 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22891488_1.fastq.gz	Fastqsanger.gz
	SRR22891489_1.fastq.gz	Fastqsanger.gz
	SRR22891489_2.fastq.gz	Fastqsanger.gz

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Project description:MicroRNAs (miRNAs) have emerged as important gene regulators and are recognized as key players in tumorigenesis. miR-143 is reported to be down-regulated in several cancers, but knowledge of its targets in colon cancer remains limited. To investigate the role of miR-143 in colon cancer, we have employed a microarray based approach to identify miR-143 targets. Based on seed site enrichment analyses and unbiased word analyses, we found a significant enrichment of miRNA binding sites in the 3’-untranslated regions (UTRs) of transcripts down-regulated upon miRNA overexpression. Here we identify Hexokinase 2 (HK2) as a direct target of miR-143 and show that re-introduction of miR-143 in the colon cancer cell line DLD-1 results in a decreased lactate secretion, indicating that miR-143 down-regulation of HK2 affects glucose metabolism in colon cancer cells.

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