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Transcriptomic signature of treatment with recombinant laminin 511-E8 and acetazolamide in human H69 cholangiocytes

ABSTRACT: Transcriptomic signature of treatment with recombinant laminin 511-E8 and acetazolamide in human H69 cholangiocytes

PROVIDER: PRJNA915951 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22892290_1.fastq.gz	Fastqsanger.gz
	SRR22892290_2.fastq.gz	Fastqsanger.gz
	SRR22892291_1.fastq.gz	Fastqsanger.gz
	SRR22892291_2.fastq.gz	Fastqsanger.gz
	SRR22892292_1.fastq.gz	Fastqsanger.gz

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Similar Datasets

Transcriptomic signature of treatment with recombinant laminin 511-E8 and acetazolamide in human H69 cholangiocytes

Project description:IgG4-related cholangitis (IRC) is the hepatobiliary manifestation of IgG4-related disease (IgG4-RD), a systemic, B-cell driven, fibroinflammatory disorder. To date, numerous autoantigens have been described including laminin 511-E8 and carbonic anhydrase (CA) enzymes (PMID 30089633, 15647194). Laminin 511-E8 is an extracellular matrix protein that has been shown to upregulate secretory components and aid in the cholangiocyte differentiation of induced pluripotent stem cells (PMID 27103433). Carbonic anhydrase enzymes catalyse the reversible hydration of carbon dioxide, thereby producing bicarbonate and a single proton. In humans there are 14 different CA isoforms with a confined subcellular distribution. All isoforms can be targeted by the pan-CA inhibitor acetazolamide, which is a registered drug for multiple indications. We aimed to investigate the role of IgG4-RD autoantigens laminin 511-E8 and CA enzymes in human cholangiocytes. Transcriptional profiling may provide insights into the underlying molecular mechanisms of these proteins on the cholangiocellular level. Thus, by RNA sequencing the transcriptomic signature of recombinant laminin 511-E8 and acetazolamide treatment was assessed in human H69 cholangiocytes.

2024-02-15 | GSE221746 | GEO

Galectin-3 and prohibitin 1 are autoantigens in IgG4-related cholangitis without protective effects against toxic bile acids

Project description:IgG4-related cholangitis (IRC) is the hepatobiliary manifestation of IgG4-related disease, a systemic B-cell-driven fibroinflammatory disorder. Four autoantigens have recently been described in IgG4-RD; annexin A11, galectin-3, laminin 511-E8, and prohibitin 1. We have previously reported a protective role of annexin A11 and laminin 511-E8 in human cholangiocytes against toxic bile acids. Here we explored a potentially protective role of the carbohydrate binding lectin galectin-3 and the scaffold proteins prohibitin 1 and 2.

2024-01-23 | PXD042856 | Pride

Affect of Aramchol on TGFβ stimulated H69 Cholangiocytes

Project description:Cholestatic liver diseases, such as primary sclerosing cholangitis (PSC), are characterized by biliary fibroinflammation. Transforming growth factor-β (TGFβ) activated cholangiocytes release signals that recruit immune cells to drive inflammation and activate myofibroblasts to deposit the extracellular matrix (ECM). TGFβ regulates stearoyl-CoA desaturase (SCD) in stimulating lipid signaling. However, the role of SCD or its inhibitor, Aramchol, has not been investigated in biliary fibroinflammation. Here we used human transformed H69 cholangiocytes that were treated with TGFβ (10ng/mL) with and without Aramchol acid (30µM) overnight. RNA-seq analysis showed significant inhibition of TGFβ-induced hepatic fibrosis pathways while upregulating peroxisome proliferator-activated receptor (PPAR) signaling by Aramchol.

2025-07-23 | GSE303012 | GEO

Affect of Aramchol on TGFβ stimulated H69 Cholangiocytes

Project description:Affect of Aramchol on TGFβ stimulated H69 Cholangiocytes

| PRJNA1293357 | ENA

RNA-seq analysis of mESCs cultured on either Laminin-511 or gelatin coated dishes in either Std or 2i medium

Project description:Some mouse embryonic stem cell (mESC) lines need to be maintained on feeder cells in gelatin/Std condition. To eliminate the need for feeder cells, we decided to maintain the C57BL6/J mESCs on dishes coated with Laminin-511 (LN511) enabling maintenance of mESCs without feeder cells in Std condition (Domogatskaya et al., 2008). To compare the transcriptomes of mESCs cultured on gelatin-coated dish and those cultured on Laminin-511 coated dish, we performed RNA-Seq analysis. We found that the transcriptomes of mESCs cultured on gelatin-coated dish and those cultured on LN511 coated dish showed no considerable difference in expression patterns.

2020-06-04 | GSE132591 | GEO

Laminin 511-E8 Fragment Offers Superior Adhesion Properties for Gastric Cancer Cells Compared with Full-Length Laminin 511.

Project description:BackgroundThe interaction between cancer cells and laminin (Ln) is a key event in tumor invasion and metastasis. Previously, we determined the effect of full-length Ln511 on gastric cancer cells. However, the interactions between the Ln511-E8 fragment, a truncated protein of Ln511, and gastric cancer cells have not been investigated.MethodsWe investigated the adhesion properties of gastric cancer cells to full-length Ln511 and Ln511-E8 fragments.ResultsThe proliferation of four gastric cancer cell lines (SH-10-TC, MKN74, SC-6-JCK, and MKN45) was highest on the Ln511-E8 fragment. Further, a larger cytoplasm was observed in SH-10-TC and MKN74 cells cultured on full-length Ln511 or Ln511-E8 fragments. The percentage of adhesive cells was highest on the Ln511-E8 fragment in all four cell lines. Moreover, adhesion of the gastric cancer cells to Ln511-E8 fragment-coated plates was reduced by the Cdc42 GTPase inhibitor in a dose-dependent manner, suggesting the involvement of Cdc42 in the Ln511-E8 fragment-induced enhanced adhesion of gastric cancer cells.ConclusionsThe Ln511-E8 fragment had a greater impact on the adhesion, morphology, and proliferation of gastric cancer cells than full-length laminin. Thus, the Ln511-E8 fragment is suitable for investigating the interaction between gastric cancer cells and extracellular matrices in tumor invasion and metastasis.

| S-EPMC9164087 | biostudies-literature

MicroRNA profiling of human cholangiocyte H69 cells after exposure to tumor necrosis factor-alpha

Project description:H69 cells (human cholangiocytes) were exposed to TNF-alpha (10 ng/ml) for 8 h and were then collected for miRNA analysis, compared with non-treated control cells.

2011-01-01 | E-MEXP-2049 | biostudies-arrayexpress

Laminin-511-E8 promotes efficient in vitro expansion of human limbal melanocytes.

Project description:Limbal melanocytes, located in the basal epithelial layer of the corneoscleral limbus, represent essential components of the corneal epithelial stem cell niche, but, due to difficulties in their isolation and cultivation, their biological roles and potential for stem cell-based tissue engineering approaches have not been comprehensively studied. Here, we established a protocol for the efficient isolation and cultivation of pure populations of human limbal melanocytes, which could be expanded at high yield by using recombinant laminin (LN)-511-E8 as culture substrate. Co-cultivation of limbal melanocytes with limbal epithelial stem/progenitor cells on fibrin hydrogels pre-incubated with LN-511-E8 resulted in multilayered stratified epithelial constructs within ten days. By reproducing physiological cell-cell and cell-matrix interactions of the native niche environment, these biomimetic co-culture systems provide a promising experimental model for investigating the functional roles of melanocytes in the limbal stem cell niche and their suitability for developing advanced epithelial grafts for ocular surface surface reconstruction.

| S-EPMC7338389 | biostudies-literature

Laminin 511-E8, an autoantigen in IgG4-related cholangitis, contributes to cholangiocyte protection

Project description: Not available

| S-EPMC10959701 | biostudies-literature

Laminin-directed fate determination of human induced pluripotent stem cells (hiPSCs) during ocular cell differentiation

Project description:The extracellular matrix plays a crucial role in stem cell maintenance, expansion and differentiation. Laminin, a basement membrane protein, is effective in supporting cultures of human pluripotent stem cells. To furhter investigate the effects of different isoforms of laminin on ectodemral differentiation of hiPSCs, we performed gene expression analysis of differentiated human iPS cells on E8 fragments of laminin isoforms (LN111E8, LN211E8, LN332E8, LN411E8, and LN511E8).

2018-10-17 | GSE116591 | GEO

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