Lens culinaris cultivar:Lentil
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ABSTRACT:
PROVIDER: PRJNA916816 | ENA |
REPOSITORIES: ENA
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Project description:Drought stress is one of the main environmental factors that affects growth and productivity of crop plants, including lentil. To gain insights into the genome-wide transcriptional regulation in lentil root and leaf under short- and long-term drought conditions, we performed RNA-seq on a drought-sensitive lentil cultivar (Lens culinaris Medik. cv. Sultan). After establishing drought conditions, lentil samples were subjected to de novo RNA-seq-based transcriptome analysis. The 207,076 gene transcripts were successfully constructed by de novo assembly from the sequences obtained from root, leaf, and stems. Differentially expressed gene (DEG) analysis on these transcripts indicated that period of drought stress had a greater impact on the transcriptional regulation in lentil root. The numbers of DEGs were 2915 under short-term drought stress while the numbers of DEGs were increased to 18,327 under long-term drought stress condition in the root. Further, Gene Ontology analysis revealed that the following biological processes were differentially regulated in response to long-term drought stress: protein phosphorylation, embryo development seed dormancy, DNA replication, and maintenance of root meristem identity. Additionally, DEGs, which play a role in circadian rhythm and photoreception, were downregulated suggesting that drought stress has a negative effect on the internal oscillators which may have detrimental consequences on plant growth and survival. Collectively, this study provides a detailed comparative transcriptome response of drought-sensitive lentil strain under short- and long-term drought conditions in root and leaf. Our finding suggests that not only the regulation of genes in leaves is important but also genes regulated in roots are important and need to be considered for improving drought tolerance in lentil.
2019-04-19 | GSE115199 | GEO
Project description:MicroRNAs (miRNAs) are key post-transcriptional regulators that influence plant development, metabolism, and stress adaptation. Despite the agronomic importance of lentil (Lens culinaris Medik.), its miRNA landscape remains poorly characterized. This study aimed to establish a comprehensive, tissue-specific atlas of conserved and novel miRNAs to improve understanding of gene regulation in lentil. Small RNA libraries from seven tissues—root, shoot, stem, mature leaf, flower, flower bud, and young pod—were sequenced using the Illumina HiSeq platform, generating over 432 million reads. A total of 1,490 miRNA candidates were identified, comprising 415 conserved and 1,075 novel miRNAs. Among 370 miRNAs with sufficient expression for analysis, 61% showed significant tissue-dependent variation, revealing extensive spatial regulation. Conserved miRNAs such as miR156, miR159, miR164, and miR172 displayed expression patterns consistent with their known developmental roles, whereas most novel miRNAs exhibited tissue-restricted accumulation, particularly in roots and reproductive organs. Predicted target and enrichment analyses indicated that conserved miRNAs primarily regulate transcription factors and hormone signaling components, while novel miRNAs are associated with redox homeostasis, lipid metabolism, and transport processes. This study provides the first comprehensive overview of miRNA expression in lentil, revealing a dual-layer regulatory architecture comprising conserved miRNAs that coordinate core developmental pathways and novel miRNAs that fine-tune tissue-specific and adaptive responses. The expanded lentil miRNA repertoire established here represents a valuable resource for future studies in legumes, including functional, evolutionary, and breeding-related applications.
2026-02-01 | GSE317753 | GEO
Project description:Lens culinaris subsp. culinaris Variation
| PRJNA528610 | ENA
Project description:Lens culinaris subsp. culinaris Variation
| PRJNA522236 | ENA
Project description:Lens culinaris cultivar:CDC Redberry Genome sequencing and assembly
| PRJNA343689 | ENA