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PRC1 directs PRC2-H3K27me3 deposition to shield adult spermatogonial stem cells from differentiation [CUT&Tag, CUT&RUN]

ABSTRACT: PRC1 directs PRC2-H3K27me3 deposition to shield adult spermatogonial stem cells from differentiation [CUT&Tag, CUT&RUN]

PROVIDER: PRJNA916894 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22938048_1.fastq.gz	Fastqsanger.gz
	SRR22938048_2.fastq.gz	Fastqsanger.gz
	SRR22938049_1.fastq.gz	Fastqsanger.gz
	SRR22938049_2.fastq.gz	Fastqsanger.gz
	SRR22938050_1.fastq.gz	Fastqsanger.gz

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PRC1 directs PRC2-H3K27me3 deposition to shield adult spermatogonial stem cells from differentiation [CUT&Tag, CUT&RUN]

Project description:To understand the functional role of PRC1 in defining the epigenomic and transcriptional landscape in Aundiff, we eliminated PRC1 function in germ cells using Ddx4-Cre to mutate the E3 ubiquitin ligase RNF2 on a background lacking its partially redundant paralog RING1 (RING1A) to create PRC1 conditional knockout (PRC1cKO) mice. We discover a key role of Polycomb complexes in shielding the undifferentiated state of mouse adult spermatogonia. CUT&RUN or CUT&Tag for H2AK119ub, H3K27me3 and H3K4me3 in WT, PRC1ctrl and PRC1cKO spermatogonial cells.

2023-12-18 | GSE221942 | GEO

PRC1 directs PRC2-H3K27me3 deposition to shield adult spermatogonial stem cells from differentiation

Project description:PRC1 directs PRC2-H3K27me3 deposition to shield adult spermatogonial stem cells from differentiation

| PRJNA916889 | ENA

PRC1 directs PRC2-H3K27me3 deposition to shield adult spermatogonial stem cells from differentiation [RNA-seq]

Project description:PRC1 directs PRC2-H3K27me3 deposition to shield adult spermatogonial stem cells from differentiation [RNA-seq]

| PRJNA916896 | ENA

PRC1 directs PRC2-H3K27me3 deposition to shield adult spermatogonial stem cells from differentiation [RNA-seq]

Project description:To understand the functional role of PRC1 in defining the epigenomic and transcriptional landscape in Aundiff, we eliminated PRC1 function in germ cells using Ddx4-Cre to mutate the E3 ubiquitin ligase RNF2 on a background lacking its partially redundant paralog RING1 (RING1A) to create PRC1 conditional knockout (PRC1cKO) mice. We discover a key role of Polycomb complexes in shielding the undifferentiated state of mouse adult spermatogonia. We then performed gene expression profiling analysis using data obtained from RNA-seq of WT, PRC1ctrl and PRC1cKO spermatogonial cells.

2023-12-18 | GSE221943 | GEO

PRC1 directs PRC2-H3K27me3 deposition to shield adult spermatogonial stem cells from differentiation.

Project description:Spermatogonial stem cells functionality reside in the slow-cycling and heterogeneous undifferentiated spermatogonia cell population. This pool of cells supports lifelong fertility in adult males by balancing self-renewal and differentiation to produce haploid gametes. However, the molecular mechanisms underpinning long-term stemness of undifferentiated spermatogonia during adulthood remain unclear. Here, we discover that an epigenetic regulator, Polycomb repressive complex 1 (PRC1), shields adult undifferentiated spermatogonia from differentiation, maintains slow cycling, and directs commitment to differentiation during steady-state spermatogenesis in adults. We show that PRC2-mediated H3K27me3 is an epigenetic hallmark of adult undifferentiated spermatogonia. Indeed, spermatogonial differentiation is accompanied by a global loss of H3K27me3. Disruption of PRC1 impairs global H3K27me3 deposition, leading to precocious spermatogonial differentiation. Therefore, PRC1 directs PRC2-H3K27me3 deposition to maintain the self-renewing state of undifferentiated spermatogonia. Importantly, in contrast to its role in other tissue stem cells, PRC1 negatively regulates the cell cycle to maintain slow cycling of undifferentiated spermatogonia. Our findings have implications for how epigenetic regulators can be tuned to regulate the stem cell potential, cell cycle and differentiation to ensure lifelong fertility in adult males.

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Mediator Subunit 30 Directs Myc Oncogenesis [Cut&Tag]

Project description:Mediator Subunit 30 Directs Myc Oncogenesis [Cut&Tag]

2023-11-08 | GSE217545 | GEO

PRC1 directs PRC2-H3K27me3 deposition to shield adult spermatogonial stem cells from differentiation

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-12-18 | GSE221944 | GEO

Mediator Subunit 30 Directs Myc Oncogenesis [Cut&Tag]

Project description:Mediator Subunit 30 Directs Myc Oncogenesis [Cut&Tag]

| PRJNA899522 | ENA

Identification of chromatin states during zebrafish gastrulation using CUT&RUN and CUT&Tag

Project description:Here we describe successful implementation of CUT&Tag for profiling protein-DNA interactions in zebrafish embryos. We optimized CUT&Tag protocol to generate high resolution maps of enrichment for the histone variant H2A.Z during zebrafish development. We were able to establish dynamics of H2A.Z genomic patterning from shield stage to 24hpf embryos. Our work demonstrates the power of combining CUT&Tag with the strengths of the zebrafish system to better understand the changing embryonic chromatin landscape and its roles in shaping development.

2022-02-15 | GSE178559 | GEO

Disruption of PRC1 Components RING1A and RING1B Promotes Angiogenesis via Relieving BMP4 Repression [CUT&Tag]

Project description:Angiogenesis is crucial for maintaining tissue homeostasis and responding to injury and disease. Epigenetic mechanisms have been elucidated to regulate the expression of pro-angiogenic factors and modulate angiogenesis. The Polycomb repressive complex 1 (PRC1) is a key epigenetic repressive complex that regulates multiple biological functions. Within this complex, RING1A and RING1B, which serve as the E3 ubiquitin ligases for PRC1, contribute to the monoubiquitylation of histone H2A on Lysine 119 (H2AK119ub), thereby repressing gene transcription. However, the role of RING1A and RING1B in regulating angiogenesis has remained elusive. In this study, we demonstrate that RING1A and RING1B suppress angiogenic capacity in vitro, although they do not inhibit migration and proliferation in endothelial cells (ECs). Furthermore, by integrating RNA sequencing data from RING1A knockdown or RING1B knockdown ECs with CUT&Tag assay results for RING1A, RING1B, and H2AK119ub in ECs, we have identified that PRC1 inhibits angiogenesis by repressing BMP4 expression, a pro-angiogenic factor known to regulate angiogenesis. Additionally, we verify that the knockdown of RING1A and RING1B enhances angiogenesis in vivo, which may provide a new therapeutic target for angiogenesis-related diseases.

2025-07-24 | GSE281870 | GEO

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