Project description:The transcriptional regulation of chlamydospore development remains poorly understood in Arthrobotrys flagrans. In model fungi, the conserved regulators LaeA, Ste12, VelB, StuA, and AbaA coordinate spore differentiation, stress responses, and secondary metabolism, yet their genome-wide targets and regulatory hierarchies in A. flagrans have never been explored. To fill this gap, we performed ChIP-seq to map the global DNA-binding profiles of these seven transcription factors during chlamydospore formation. Our goal is to decode the regulatory circuitry governing this process, identify direct target genes, and elucidate the transcriptional cascades that drive spore morphogenesis and stress adaptation.
Project description:To investigate the molecular mechanisms underlying chlamydospore formation in Arthrobotrys flagrans, this study conducted proteomic analyses of gene knockout strains.
Project description:Next Generation Sequencing analysis to examine the transcriptional responses of the nematode-trapping fungus Duddingtonia flagrans with the treatment of extraction of Caenorhabditis elegans