Project description:Transcriptional profiling of Mycobacterium smegmatis comparing strains undergoing I-SceI generated DNA damage at a single genomic locus Gene designations are the updated annotation
Project description:Compare the gene expression difference between MSMEG_2225 knockout strains and wild-type strains by RNA sequencing in Mycobacterium smegmatis. The goal of this study is that detection MSMEG_2225 acts as a transcriptional factor and regulates the target genes expression.
Project description:Compare the gene expression difference between hpoR knockout strains and wild-type strains by RNA sequencing in Mycobacterium smegmatis. The goal of this study is that detection HpoR acts as a transcriptional factor and regulates the target genes expression.
Project description:Compare the gene expression difference between ltmA knockout strains and wild-type strains by RNA sequencing in Mycobacterium smegmatis. The goal of this study is that detection LtmA acts as a transcriptional factor and regulates the target genes expression.
2018-07-02 | GSE109224 | GEO
Project description:Mutational analysis of antibiotic resistant strains of Mycobacterium smegmatis
Project description:CarD is an essential transcription factor for Mycobacterium smegmatis. We identified a new interaction partner of CarD and named it CrsL (MSMEG_5890). To address the genome-wide effect of CrsL, we generated knockdown strains for both carD (MSMEG_6077) and crsL (MSMEG_5890) genes in Mycobacterium smegmatis. We aimed to compare the differentially expressed genes of crsL knockdown and correlate them with the ones of carD knockdown to describe the role of CrsL in transcription.
Project description:CarD is essential transcription factors in mycobacteria. For ChIP-seq, we used Mycobacterium smegmatis strains with CarD-FLAG (LK2539), where an additional copy of CarD under anhydrotetracycline inducible promoter was stably inserted into the genome. CarD-FLAG was expressed in exponential phase and ChIP performed at mid-exponential phase of growth.
Project description:Proteome comparison of two Mycolicibacterium smegmatis strains, mc2155 and the recombinant strain expressing MTS1338, a small non-coding RNA of Mycobacterium tuberculosis. The recombinant strain was obtained by electroporation of MTS1338-expressing plasmid into M. smegmatis mc2155 cells
Project description:RbpA is an essential transcription factors in mycobacteria. For ChIP-seq, we used Mycobacterium smegmatis strains with RbpA-FLAG (LK2541), where an additional copy of RbpA under anhydrotetracycline inducible promoter was stably inserted into the genome. RbpA-FLAG was expressed in exponential phase and ChIP performed at mid-exponential phase of growth.
