Project description:Transcriptional profiling of Mycobacterium smegmatis comparing strains undergoing I-SceI generated DNA damage at a single genomic locus Gene designations are the updated annotation
Project description:Compare the gene expression difference between hpoR knockout strains and wild-type strains by RNA sequencing in Mycobacterium smegmatis. The goal of this study is that detection HpoR acts as a transcriptional factor and regulates the target genes expression.
Project description:Compare the gene expression difference between ltmA knockout strains and wild-type strains by RNA sequencing in Mycobacterium smegmatis. The goal of this study is that detection LtmA acts as a transcriptional factor and regulates the target genes expression.
Project description:Compare the gene expression difference between MSMEG_2225 knockout strains and wild-type strains by RNA sequencing in Mycobacterium smegmatis. The goal of this study is that detection MSMEG_2225 acts as a transcriptional factor and regulates the target genes expression.
2021-02-08 | GSE110385 | GEO
Project description:Mutational analysis of antibiotic resistant strains of Mycobacterium smegmatis
Project description:Proteome comparison of two Mycolicibacterium smegmatis strains, mc2155 and the recombinant strain expressing MTS1338, a small non-coding RNA of Mycobacterium tuberculosis. The recombinant strain was obtained by electroporation of MTS1338-expressing plasmid into M. smegmatis mc2155 cells
Project description:The experiment was carried out to study the effect of loss of (p)ppGpp or cyclic di-GMP on the gene expression pattern of Mycobacterium smegmatis MC2 155 strains. The cells were cultured in MB7H9 broth containing 0.2% glycerol and 0.05% Tween 80. The transcriptome analysis was performed using GeneSpring GX12 software. Fold changes were calculated with respect to the median expression of wild type replicates. The number of differentially expressed genes (p <0.1) in rel knockout were 417 whereas those in dcpA knockout were 149. Earlier studies had shown that rel knockout and dcpA knockout had shown lower levels of glycopeptidolipids and polar lipids in the cell wall. The microarray data also corraborates our current findings. Organism : Mycobacterium smegmatis, Agilent Custom Mycobacterium smegmatis Gene Expression M.smegmatis_gxp_8X15K (AMADID: 029929) designed by Genotypic Technology Private Limited.
Project description:Transcriptional profiling of Mycobacterium smegmatis comparing strains undergoing I-SceI generated DNA damage at a single genomic locus Gene designations are the updated annotation Four comparisons were made, all with log phase cultures and no ATc added. Darr Site(+) with an empty vector compared to wild-type M. smegmatis Site(+), Darr Site(+) with a vector expressing WT Arr compared to wild-type M. smegmatis Site(+), Darr Site(+) with a vector expressing ArrH18A compared to wild-type M. smegmatis Site(+), Darr Site(+) with a vector expressing Arr D83A compared to wild-type M. smegmatis Site(+). 3 biological replicates of each strain for each experiment.
Project description:Understanding emergence of drug resistance in pathogens systematically is a major challenge. To address this issue, lab-evolved strain resistant to first-line tuberculosis drug, isoniazid (INH), was analysed. Transcriptome analysis of different conditions revealed genes that are differentially regulated in wild-type (WT) and isoniazid resistant (2XR and 4XR) strains. Microarray analysis was performed for WT, sub-MIC, 2XR and 4XR strains of Mycobacterium smegmatis. Overall, the numbers of DEGs observed are 1529 in sub-MIC (596 - up, 933 - down), 1381 (899 – up, 482 – down) in 2XR and 716 in 4XR (267 – up, 449 – down) conditions. 52 of them are seen to be up-regulated across all drug concentrations as compared to WT while 185 are seen to be down-regulated in all. Transcriptome analysis revealed that there are variations in gene expression pattern in the resistant strains as compared to WT strain. This indicates that differential regulation of genes is, in some way, responsible for conferring the phenotype to the organism.
