Conticribra weissflogii
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ABSTRACT:
PROVIDER: PRJNA922708 | ENA |
REPOSITORIES: ENA
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Project description:Conticribra weissflogii strain:CCMP1051 Transcriptome or Gene expression
| PRJNA288683 | ENA
Project description:Conticribra weissflogii strain:CCMP 1336 Genome sequencing and assembly
| PRJNA665191 | ENA
Project description:Physiological trade-off and transcriptome reprogramming are involved in acclimation to salinity gradient in diatoms
| PRJNA298677 | ENA
Project description:Background: Fucoxanthin (FX), a xanthophyll pigment which occurs in marine brown algae with remarkable biological properties, has been proven to be safe for consumption by animals. Although FX has various pharmacological effects including anti-inflammatory, anti-tumor, anti-obesity, antioxidant, anti-diabetic, anti-malarial, and anti-lipid, in vivo protective effect against sepsis has not been reported. In this study, we aimed at evaluation the efficacy of the FX in a model of sepsis mouse. Methods: FX was successfully isolated from Conticribra weissflogii ND-8 for the first time. The FX was identified by thin-layer chromatography (TLC), high-performance liquid chromatography-mass spectrometry (HPLC-MS), and nuclear magnetic resonance (NMR). Animals were randomly divided into 9 groups, including Sham group (mouse received an intraperitoneal injection of normal saline 1.0 ml/kg), FX-treated (0.1-1.0 ml/kg), Lipopolysaccharide (LPS)-treated (20 mg/kg), FX+LPS-treated (0.1-10.0 mg/kg and 20 mg/kg, respectively), and urinastatin groups (104 U/kg). Nuclear factor (NF)-κB activation could be potential treatment for sepsis. NF-κB signaling components were determined by western-blotting. IL-6, IL-1β, TNF-α production, and NF-κB activation were evaluated by ELISA and immunofluorescent staining in vitro. Results: FX was found to decrease the expression of inflammatory cytokines including IL-6, IL-1β, and TNF-α, in a prophylactic manner in the LPS-induced sepsis mouse model. Meanwhile, FX significantly inhibits phosphorylation of the NF-κB signaling pathway induced by LPS at the cellular level and reduces the nuclear translocation of NF-κB. The IC50 for suppressing the expression of NF-κB was 11.08 ± 0.78 μM in the THP1-Lucia™ NF-κB cells. Furthermore, FX also inhibits the expression of inflammatory factors in a dose-dependent manner with the IC50 inhibition of IL-6 production was 2.19 ± 0.70 μM in Raw267.4 macrophage cells. It is likely that the molecules with the ability of targeting NF-κB activation and inflammasome assembly, such as fucoxanthin, are interesting subjects to be used for treating sepsis.
| S-EPMC6722224 | biostudies-literature
Project description:Effects of low light and iron/light co-limitation on Thalassiosira weissflogii
| PRJEB12400 | ENA
Project description:The production of fucoxanthin and fatty acids in Conticribra weissflogii has been examined, but the role of elements like phosphorus in their mutualistic interactions is not well understood. To fill this gap, our study utilized potassium dihydrogen phosphate (KH2PO4) as a source of phosphorus to examine its impact on the synthesis of fucoxanthin and fatty acids in C. weissflogii. Our findings revealed that at a phosphorus concentration of 10 mg L-1, the cell density (9.5 × 105 cells mL-1), carotenoid concentration (1.67 mg g-1), fucoxanthin concentration (0.91 mg L-1), and fucoxanthin content (1.33 mg g-1) were maximized. Additionally, at a phosphorus concentration of 20 mg L-1, cell dry weight (0.76 ± 0.08 g L-1), total fatty acid content, saturated fatty acids, and unsaturated fatty acids were all at their highest levels, making this concentration optimal for EPA accumulation. In conclusion, manipulating the phosphorus concentration can enhance the levels of fucoxanthin and unsaturated fatty acids in C. weissflogii, offering valuable insights into the co-production of these two high-value compounds within this species.
| S-EPMC11678120 | biostudies-literature