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PROVIDER: PRJNA922994 | ENA |
REPOSITORIES: ENA
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Project description:Genome sequencing of gray short-tailed opossum (Monodelphis domestica)
| PRJNA1139788 | ENA
Project description:Monodelphis domestica (gray short-tailed opossum) genome, mMonDom1, primary haplotype
| PRJNA915592 | ENA
Project description:Monodelphis domestica (gray short-tailed opossum) genome, mMonDom1, alternate haplotype
| PRJNA915591 | ENA
Project description:Gray, short-tailed opossum (laboratory opossum)
| PRJNA12560 | ENA
Project description:Marsupials have been a powerful comparative model to understand mammalian biology. However, because of the unique characteristics of their embryology, marsupial pluripotency architecture remains to be fully understood, and nobody has succeeded in developing embryonic stem cells (ESCs) from any marsupial species. We have developed an integration-free induced pluripotent stem cell (iPSC) reprogramming method and established validated iPSC lines from two fully inbred strains of the gray short-tailed opossum (Monodelphis domestica). A comprehensive characterization of the M. domestica skin fibroblasts and their reprogrammed iPSCs was performed by genome-wide mRNA sequencing. The established monoiPSCs showed a significant (6,181 DE genes) but highly uniform (between clone r2 at 95% CI = 0.973 ± 0.007) resetting of the cellular transcriptome during reprogramming and were highly similar to eutherian ESCs and iPSCs in their overall transcriptomic and functional profiles. However, monoiPSCs showed unique regulatory architecture of the core pluripotency transcription factors and were more like epiblasts. Our results suggest POU5F1 and the splice variant specific expression of POU5F3 synergistically regulate the opossum pluripotency gene network. It is plausible that POU5F1, POU5F3 splice variant XM_016427856.1, and SOX2 form a self-regulatory network. NANOG expression, however, was specific to monoiPSCs and epiblasts, and displayed a distinct expression profile in embryonic cells. Furthermore, POU5F1 was highly expressed in trophectoderm cells, whereas all other pluripotency transcription factors were significantly downregulated, suggesting that the regulatory architecture of core pluripotency genes of marsupials may be distinct from that of eutherians.
2022-11-02 | GSE206122 | GEO
Project description:This experiment contains the subset of data corresponding to gray short-tailed opossum RNA-Seq data from experiment E-GEOD-30352 (http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-30352/), which goal is to understand the dynamics of mammalian transcriptome evolution. To study mammalian transcriptome evolution at high resolution, we generated RNA-Seq data (∼3.2 billion Illumina Genome Analyser IIx reads of 76 base pairs) for the polyadenylated RNA fraction of brain (cerebral cortex or whole brain without cerebellum), cerebellum, heart, kidney, liver and testis (usually from one male and one female per somatic tissue and two males for testis) from nine mammalian species: placental mammals (great apes, including humans; rhesus macaque; mouse), marsupials (gray short-tailed opossum) and monotremes (platypus). Corresponding data (∼0.3 billion reads) were generated for a bird (red jungle fowl, a non-domesticated chicken) and used as an evolutionary outgroup.
2011-10-12 | E-MTAB-3719 | biostudies-arrayexpress
Project description:The OK cell line derived from kidney of a female opossum Didelphys virginiana has proven to be a useful model in which to investigate the unique regulation of ion transport and membrane trafficking mechanisms in the proximal tubule (PT). Sequence data and comparison of the transcriptome of this cell line to eutherian mammal PTs would further broaden the utility of this culture model. However, genomic sequence for Didelphys virginiana is not available and although a draft genome sequence for the opossum Monodelphis domestica (sequenced in 2012 by the Broad Institute) exists, its relatedness and similarity of the transcriptome to the Didelphys virginiana species is not known. The Monodelphis domestica sequence is not highly annotated, and the majority of transcripts are predicted rather than experimentally validated. Using deep RNA sequencing of the Didelphys virginiana OK cell line we characterized its transcriptome using de novo transcriptome assembly and alignment to the Monodelphis domestica genome. The quality of the de novo assembled transcriptome was assessed by the extent of homology to sequences in nucleotide and protein databases. Gene expression levels in the OK cell line, from both the de novo transcriptome and genes aligned to the Monodelphis domestica genome, were compared to publicly available rat kidney nephron segment expression data. Our studies demonstrate the expression in OK cells of numerous PT specific ion transporters and other key proteins relevant for rodent and human PT function. The sequence and expression data reported here provide a new and important resource for studies on the regulation of PT mRNA and protein expression.
2017-08-07 | GSE94443 | GEO
Project description:The brief placental attachment of the gray short-tailed opossum Monodelphis domestica spans only the final two days of the 14-day pregnancy. During this time the fetus grows rapidly and a pronounced spike in inflammatory signaling occurs. It was recently discovered that these cytokines are produced primarily by fetal cells, the syncytial knots which develop at the end stage of placentation after day 13 of gestation. To investigate this further, we conducted laser microdissection on placental membranes (omphalopleure) at 13.5 days post copulation to compare to published transcriptomes at 12.5 days post copulation. Analysis revealed indeed a pronounced expression of inflammatory cytokines in the 13.5 samples. We hypothesized that placental cytokines act as solicitation signals benefitting the fetus by effects on maternal nutrient transfer. To test this, we administered anti-inflammatory drugs to pregnant opossums during the attachment phase targeted at three arms of this response: IL1A via the receptor inhibitor anakinra, IL6 via the receptor inhibitor tocilizumab, and prostaglandin E2 via the PTGS2 synthetase inhibitor meloxicam. Inhibition of IL-1 and IL-6 signaling resulted in significant increases in fetal biomass, suggesting that they impose a cost to biomass acquisition by offspring rather than acting in direct solicitation. However, in the case of anakinra, saline-treated control individuals showed a greater mean surviving litter size compared to IL-1-inhibited animals. Together, our results suggest that marsupial end-pregnancy inflammation has acquired a novel function modulating both fetal growth and survival during pregnancy.
2026-01-31 | GSE302731 | GEO
Project description:Evidence from a few genes of diverse species suggests that marsupial X-chromosome inactivation (XCI) is characterized by exclusive, but leaky, inactivation of the paternally derived X chromosome. To comprehensively study the mechanism of marsupial XCI, we profiled parent-of-origin-specific-allele expression, DNA methylation, and histone modifications in opossum fetal brain and extra-embryonic membranes. The majority (152/176) of X-linked genes exhibited paternally imprinted expression with nearly 100% maternal allele expression, whereas 24 loci (14%) escaped inactivation showing varying levels of biallelic expression. In addition to regulation by the non-coding RSX transcript, strong depletion of H3K27me3 at escaper gene loci indicates that histone states also influence opossum XCI. Notably, the opossum does not show an association between X-linked gene expression and promoter DNA methylation. Our study provides the first comprehensive catalogue of parent-of-origin expression status for X-linked genes in a marsupial and sheds light on the regulation and evolution of imprinted XCI in mammals. Profiling of four histone modifications in embryonic day 13 opossum (Monodelphis domestica) fetal brain by Illumina ChIP-seq
2013-09-23 | E-GEOD-45186 | biostudies-arrayexpress