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Chromatin interaction of Cebpa during adipocyte differentiation (4C-Seq)

ABSTRACT: Chromatin interaction of Cebpa during adipocyte differentiation (4C-Seq)

PROVIDER: PRJNA923020 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23054445.fastq.gz	Fastqsanger.gz
	SRR23054446.fastq.gz	Fastqsanger.gz
	SRR23054447.fastq.gz	Fastqsanger.gz
	SRR23054448.fastq.gz	Fastqsanger.gz
	SRR23054449.fastq.gz	Fastqsanger.gz

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Chromatin interaction of Cebpa during adipocyte differentiation (4C-Seq)

Project description:Cebpa is a critical transcription factor gene for adipocyte differentiation and adipose tissue development. However, mechanisms controlling Cebpa expression during adipogenic differentiation remain largely unknown. Here, we generated the high-resolution chromatin interaction maps of Cebpa in 3T3-L1 preadipocytes (3T3-L1) and 3T3-L1 adipocytes (3T3-L1-AD) using circularized chromosome conformation capture coupled with next-generation sequencing (4C-seq), and characterized differences in their chromatin interactomes and chromatin status of the interaction sites during adipogenic differentiation. We performed a 4C-seq experiment on inguinal white adipose tissue (iWAT) to evaluate whether chromatin interaction between Cebpa-L1-AD-En2 and Cebpa promoters in 3T3-L1 adipocytes also exists in mouse adipose tissue.

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