Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Increased chromatin accessibility promotes the evolution of a transcriptional silencer in Drosophila [greenCUT&RUN]

ABSTRACT: Increased chromatin accessibility promotes the evolution of a transcriptional silencer in Drosophila [greenCUT&RUN]

PROVIDER: PRJNA923260 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR23060910_1.fastq.gz	Fastqsanger.gz
	SRR23060910_2.fastq.gz	Fastqsanger.gz
	SRR23060911_1.fastq.gz	Fastqsanger.gz
	SRR23060911_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 4 of 4

Similar Datasets

Increased chromatin accessibility promotes the evolution of a transcriptional silencer in Drosophila [CUT&RUN]

Project description:Increased chromatin accessibility promotes the evolution of a transcriptional silencer in Drosophila [CUT&RUN]

| PRJNA923258 | ENA

Increased chromatin accessibility promotes the evolution of a transcriptional silencer in Drosophila.

Project description:Increased chromatin accessibility promotes the evolution of a transcriptional silencer in Drosophila.

| PRJNA923253 | ENA

Increased chromatin accessibility promotes the evolution of a transcriptional silencer in Drosophila [ATAC-seq]

Project description:Increased chromatin accessibility promotes the evolution of a transcriptional silencer in Drosophila [ATAC-seq]

| PRJNA923257 | ENA

Increased chromatin accessibility promotes the evolution of a transcriptional silencer in Drosophila [greenCUT&RUN]

Project description:The loss of discrete morphological traits, the most common evolutionary transition, is typically driven by changes in expression of developmental genes. Mutations accumulating in regulatory elements of these genes can disrupt DNA binding sites for transcription factors patterning their spatial expression, or delete entire enhancers. Regulatory elements, however, may in principle be silenced through other mechanisms, including changes in chromatin accessibility, or the emergence of repressive elements. Here, we show that an increase in chromatin accessibility at the pigmentation gene yellow, combined with the gain of a repressor site, underlie the evolutionary loss of a spot pigmentation pattern on the wings of a Drosophila species. The evolutionary gain of accessibility of this repressive element is regulated by E93, a transcription factor governing the progress of metamorphosis. This convoluted evolutionary scenario contrasts with the classical parsimonious mutational paths generally envisioned and often documented for morphological losses. It illustrates for the first time how evolutionary changes in chromatin accessibility may directly contribute to morphological diversification.

2023-02-17 | GSE222716 | GEO

Increased chromatin accessibility promotes the evolution of a transcriptional silencer in Drosophila [CUT&RUN]

2023-02-17 | GSE222715 | GEO

Increased chromatin accessibility promotes the evolution of a transcriptional silencer in Drosophila [ATAC-seq]

2023-02-17 | GSE222714 | GEO

Increased chromatin accessibility promotes the evolution of a transcriptional silencer in <i>Drosophila</i>.

Project description:The loss of discrete morphological traits, the most common evolutionary transition, is typically driven by changes in developmental gene expression. Mutations accumulating in regulatory elements of these genes can disrupt DNA binding sites for transcription factors patterning their spatial expression, or delete entire enhancers. Regulatory elements, however, may be silenced through changes in chromatin accessibility or the emergence of repressive elements. Here, we show that increased chromatin accessibility at the gene yellow, combined with the gain of a repressor site, underlies the loss of a wing spot pigmentation pattern in a Drosophila species. The gain of accessibility of this repressive element is regulated by E93, a transcription factor governing the progress of metamorphosis. This convoluted evolutionary scenario contrasts with the parsimonious mutational paths generally envisioned and often documented for morphological losses. It illustrates how evolutionary changes in chromatin accessibility may directly contribute to morphological diversification.

| S-EPMC9937571 | biostudies-literature

Aging is associated with increased chromatin accessibility and reduced polymerase pausing in liver [CUT&RUN]

Project description:Aging is associated with increased chromatin accessibility and reduced polymerase pausing in liver [CUT&RUN]

| PRJNA862528 | ENA

Increased chromatin accessibility promotes the evolution of a transcriptional silencer in Drosophila.

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-02-17 | GSE222717 | GEO

A tissue dissociation method optimized for ATAC-seq and CUT&RUN in Drosophila pupal tissues [CUT&RUN]

Project description:Chromatin accessibility, histone modifications and transcription factor binding are highly dynamic during Drosophila metamorphosis and drive global changes in gene expression as larval tissues differentiate into adult structures. Unfortunately, the presence of pupa cuticle on many Drosophila tissues during metamorphosis prevents enzyme access to cells and has limited the use of enzymatic in situ methods for assessing chromatin accessibility and histone modifications. Here, we present a dissociation method for cuticle-bound pupal tissues that is optimized for use with ATAC-Seq and CUT&RUN to interrogate chromatin accessibility and histone modifications. We show this method provides comparable chromatin accessibility data to the non-enzymatic approach FAIRE-seq, with only a fraction of the amount of input tissue required. This approach is also compatible with CUT&RUN, which allows genome-wide mapping of histone modifications with less than 1/10th of the tissue input required for more conventional approaches such as Chromatin Immunoprecipitation Sequencing (ChIP-seq). Our protocol makes it possible to use newer, more sensitive enzymatic in situ approaches to interrogate gene regulatory networks during Drosophila metamorphosis.

2022-08-14 | GSE211043 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data