Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Exploration of specific gene expression behind the intercellular heterogeneity of type 2 cytokine secretion activity of Group 2 innate lymphoid cell (ILC2) isolated from human peripheral blood

ABSTRACT: Exploration of specific gene expression behind the intercellular heterogeneity of type 2 cytokine secretion activity of Group 2 innate lymphoid cell (ILC2) isolated from human peripheral blood

PROVIDER: PRJNA923342 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR23068261_1.fastq.gz	Fastqsanger.gz
	SRR23068261_2.fastq.gz	Fastqsanger.gz
	SRR23068262_1.fastq.gz	Fastqsanger.gz
	SRR23068262_2.fastq.gz	Fastqsanger.gz
	SRR23068263_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 30

Similar Datasets

Exploration of specific gene expression behind the intercellular heterogeneity of type 2 cytokine secretion activity of Group 2 innate lymphoid cell (ILC2) isolated from human peripheral blood

Project description:To investigate the gene expression underlying the intercellular diversity in type 2 cytokine secretion activity of ILC2, we performed time-series measurements of type 2 cytokine secretion activity of ILC2 from human peripheral blood after IL-2/IL-33/TSLP stimulation . Single cells were collected separately before stimulation, in transition state or activated cells after stimulation, and performed RNA-seq on each cell for gene expression profiling analysis.

2023-09-13 | GSE222761 | GEO

Exploration of specific gene expression behind the intercellular heterogeneity of type 2 cytokine secretion activity of Group 2 innate lymphoid cell (ILC2) isolated from peripheral blood of severe asthma patients I

Project description:To investigate the gene expression underlying the intercellular diversity in type 2 cytokine secretion activity of ILC2, we performed time-series measurements of type 2 cytokine secretion activity of ILC2 from the peripheral blood of severe asthma patients after IL-33 stimulation . Single cells were collected separately before stimulation and secretion-negative or -positive cells after stimulation, and performed RNA-seq on each cell for gene expression profiling analysis.

2023-09-13 | GSE213810 | GEO

Project description:Exploration of specific gene expression behind the intercellular heterogeneity of type 2 cytokine secretion activity of Group 2 innate lymphoid cell (ILC2) isolated from peripheral blood of severe asthma patients I

| PRJNA882847 | ENA

Disturbed LTB4 metabolism in ILC2 Aggravates Ulcerative Colitis via intercellular ATP accumulation

Project description:LTB4 biosynthesis is critically required for cytokine secretion by the group 2 innate lymphoid cell (ILC2), though its underlying regulatory mechanisms remain incompletely characterized. To investigate how LTB4 governs ILC2 functionality, we performed RNA sequencing on sorted murine colonic ILC2s treated with or without SC-57461A—an inhibitor targeting LTA4H, the key enzyme in LTB4 anabolism. This dataset delineates transcriptomic profiles of ILC2 with pharmacologically attenuated versus intact LTB4 synthesis. Here, we identify significant alterations in mitochondria-associated genes (including Fdps, Hmgcs1, and Acat3) upon suppression of LTB4 anabolism in ILC2. These genes encode proteins essential for mitochondrial function and are indispensable for ILC2 energy metabolism. These findings collectively demonstrate that LTB4 synthesis orchestrates mitochondrial functionality in ILC2, thereby modulating their bioenergetic capacity.RNA sequencing was performed for the SC-57461A- or vehicle-treated ILC2 sorted from the large intestine of C57BL/6 mice (n = 4 per group).

2025-08-12 | GSE304475 | GEO

Exploration of the activation process of Group 2 innate lymphoid cell (ILC2) isolated from mouse fat tissues to produce type 2 cytokine [mouse]

Project description:To investigate the gene expression underlying the activation process of ILC2, we performed time-series measurements of type 2 cytokine secretion activity (IL-5 or IL-13) of ILC2 from mouse fat tissues after IL-2/IL-33 stimulation . Single cells were collected separately before stimulation (Pre-stimulation), just after exhibiting type 2 cytokine secretion (Transition state), and secretion-positive (Activated state) or secretion-negative (Silent state) after sufficient time from stimulation, and performed RNA-seq on each cell for gene expression profiling analysis.

2023-09-13 | GSE222762 | GEO

Amino acid availability acts as a metabolic rheostat to determine the magnitude of ILC2 responses - WT ILC2

Project description:Group 2 innate lymphoid cells (ILC2) are functionally poised, tissue-resident lymphocytes that respond rapidly to damage and infection at mucosal barrier sites. ILC2 reside within complex microenvironments where they are subject to cues from both the diet and invading pathogens – including helminths. Emerging evidence suggests ILC2 are acutely sensitive not only to canonical activating signals, but also perturbations in nutrient availability. In the context of helminth infection, we identify amino acid availability as a nutritional cue in regulating ILC2 responses. ILC2 were found to be uniquely pre-primed to import amino acids via the large neutral amino acid transporters Slc7a5 and Slc7a8. Cell-intrinsic deletion of these transporters individually impaired ILC2 expansion, while concurrent loss of both transporters markedly impaired the proliferative and cytokine producing capacity of ILC2. Moreover, amino acid determined the magnitude of ILC2 responses in part via tuning of mTOR. These findings implicate essential amino acids as a metabolic requisite for optimal ILC2 responses within mucosal barrier tissues.

2022-12-22 | PXD038792 | Pride

Disturbed LTB4 metabolism in ILC2 Aggravates Ulcerative Colitis via intercellular ATP accumulation

Project description:Disturbed LTB4 metabolism in ILC2 Aggravates Ulcerative Colitis via intercellular ATP accumulation

| PRJNA1301361 | ENA

Human ILC2 cells stimulated with IL-25, IL-33, or TSLP

Project description:ILC2 cells are a newly described cell type whose biology and contribution to disease are poorly understood. ILC2 cells are activated by allergens, viral infection, and/or epithelial damage via IL-33 and IL-25. ILC2 cells require IL-2, IL-7, IL-25 and IL-33 for their survival and expansion. In mice, ILC2s produce multiple mediators primarily associated with type 2 inflammation (IL-13, IL-5, IL-4, IL-6, IL-9, IL-10, GM-CSF, amphiregulin). ILC2 cells may contribute to the pathology of asthma through multiple mediators that include IL-13-independent pathways. Our goal is to compare transcriptional profiles of IL-33- or IL-25-activated ILC2 cells from blood to characterize these cells and to identify marker(s) that can be utilized to detect them in human tissue. ILC2 cells (Lineage negative, CRTH2+, CD161+, CD127+) were purified from human blood of 5 different donors by flow cytometry. The ILC2 yield ranged from 20,000 to 165,000 cells per donor (0.001-0.008% WBC). Purified ILC2s were expanded in vitro in the presence of IL-2, IL-7, IL-33 and IL-25 (each at 50 ng/ml) for 7-10 days. Expanded cells maintained the ILC2 phenotype (Lineage negative, CRTH2+, CD161+, CD127+). The cells were rested for 2 days in the presence of 1 ng/ml IL-2 and IL-7 and then treated in the presence of 1 ng/ml IL-2 and IL-7 with either media control, IL-25 (50 ng/ml), IL-33 (50 ng/ml), and/or TSLP (50 ng/ml) in combination, for 6 or 24 hours. Whole RNA was isolated via the RNeasy kit (Qiagen). Stratagene Universal Human Reference RNA was used as the reference.

2013-12-31 | E-GEOD-45458 | biostudies-arrayexpress

Expression data of ILC2 cells from Ets1-deleted and littermate control mice

Project description:The ETS1 transcription factor is required for the development and cytokine-induced expansion of ILC2 ILC2 cells isolated from ETS1-deleted or litter mate control mice were cultured on OP9-DL1 with IL-7 and IL-33. Subsequently, RNA from ICOS+ cells was extracted, labelled and hybridized to Affymetrix microarrays. The goal of this study is to investigate ETS1-dependent genes in developing ILC2 cells.

2016-04-01 | E-GEOD-79742 | biostudies-arrayexpress

Amino acid availability acts as a metabolic rheostat to determine the magnitude of ILC2 responses - SLC7A5 and SLC7A8 KO

2022-12-22 | PXD038786 | Pride