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Differential binding of PARP-1 domains (ZNI-YFP ChIP-seq)

ABSTRACT: Differential binding of PARP-1 domains (ZNI-YFP ChIP-seq)

PROVIDER: PRJNA923687 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR23077609_1.fastq.gz	Fastqsanger.gz
	SRR23077609_2.fastq.gz	Fastqsanger.gz
	SRR23077610_1.fastq.gz	Fastqsanger.gz
	SRR23077610_2.fastq.gz	Fastqsanger.gz
	SRR23077611_1.fastq.gz	Fastqsanger.gz

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Differential binding of PARP-1 domains (DZNI-YFP ChIP-seq)

Project description:We interrogated the differential binding of PARP-1 domains (ZnI, ZnII, AD-WGR) and a ZnI mutant in Drosophila via ChIP-seq of third-instar larvae.

2023-05-25 | GSE222865 | GEO

Differential binding of PARP-1 domains (AW-YFP ChIP-seq)

Project description:We interrogated the differential binding of PARP-1 domains (ZnI, ZnII, AD-WGR) and a ZnI mutant in Drosophila via ChIP-seq of third-instar larvae.

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Differential binding of PARP-1 domains (ZNII-YFP ChIP-seq)

Project description:We interrogated the differential binding of PARP-1 domains (ZnI, ZnII, AD-WGR) and a ZnI mutant in Drosophila via ChIP-seq of third-instar larvae.

2023-05-25 | GSE222875 | GEO

Differential binding of PARP-1 domains (ZNI-YFP ChIP-seq)

Project description:We interrogated the differential binding of PARP-1 domains (ZnI, ZnII, AD-WGR) and a ZnI mutant in Drosophila via ChIP-seq of third-instar larvae.

2023-05-25 | GSE222873 | GEO

Differential binding of PARP-1 domains

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-05-25 | GSE222877 | GEO

Depletion of PARP-2 in HepG2 cells

Project description:Poly(ADP-ribose) polymerase-2 (PARP-2) is acknowledged as a DNA repair enzyme; however, recently metabolic properties had been attributed to it. Hereby, we examined the metabolic consequences of PARP-2 ablation in liver. Microarray analysis of PARP-2 knockdown HepG2 cells revealed the dysregulation of lipid and cholesterol metabolism genes. Induction of cholesterol biosynthesis genes stemmed from the enhanced expression of sterol-regulatory element binding protein (SREBP)-1. We revealed that PARP-2 is a suppressor of the SREBP-1 promoter, therefore ablation of PARP-2 induces SREBP-1 expression and consequently cholesterol synthesis. PARP-2-/- mice had higher SREBP-1 expression that was translated into enhanced hepatic and serum cholesterol levels. PARP-2 silencing was performed employing shPARP-2 (small hairpin) and scPARP-2 (scrambled) shRNA by lentiviral delivery (Sigma) using 40 MOI lentiviruses coding shRNA sequence against PARP-2.

2013-12-31 | E-GEOD-43981 | biostudies-arrayexpress

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