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Regulation of human germline commitment by DMRT1

ABSTRACT: Regulation of human germline commitment by DMRT1

PROVIDER: PRJNA924734 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17208800_1.fastq.gz	Fastqsanger.gz
	SRR17208800_2.fastq.gz	Fastqsanger.gz
	SRR17208801_1.fastq.gz	Fastqsanger.gz
	SRR17208801_2.fastq.gz	Fastqsanger.gz
	SRR17208802_1.fastq.gz	Fastqsanger.gz

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Project description:Germline commitment following primordial germ cell (PGC) specification during early human development establishes an epigenetic programme and competence for gametogenesis. Here we follow the progression of nascent PGC-like cells derived from human embryonic stem cells in vitro. We show that switching from BMP signalling for PGC specification to Activin A and retinoic acid resulted in DMRT1 and CDH5 expression, the indicators of migratory PGCs in vivo. Moreover, the induction of DMRT1 and SOX17 in PGC-like cells promoted epigenetic resetting with striking global enrichment of 5-hydroxymethylcytosine and locus-specific loss of 5-methylcytosine at DMRT1 binding sites and the expression of DAZL representing DNA methylation-sensitive genes, a hallmark of the germline commitment programme. We provide insight into the unique role of DMRT1 in germline development for advances in human germ cell biology and in vitro gametogenesis.

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