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Effect of Usp17l knockdown on 2c gene in mouse embryonic stem cells (RNA-Seq)

ABSTRACT: Effect of Usp17l knockdown on 2c gene in mouse embryonic stem cells (RNA-Seq)

PROVIDER: PRJNA924793 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23100089_1.fastq.gz	Fastqsanger.gz
	SRR23100089_2.fastq.gz	Fastqsanger.gz
	SRR23100090_1.fastq.gz	Fastqsanger.gz
	SRR23100090_2.fastq.gz	Fastqsanger.gz
	SRR23100091_1.fastq.gz	Fastqsanger.gz

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Effect of Usp17l knockdown on 2c gene in mouse embryonic stem cells (RNA-Seq)

Project description:In order to study the regulation of Usp17l on 2c gene, we established Usp17l knockdown cell lines KD4 and KD9. shRNA targets the common regions of the five subtypes of Usp17l We then performed gene expression profiling with the data obtained from the RNA-seq of the 7th generation cells.

2025-01-16 | GSE223067 | GEO

Usp17l affects 2c gene transcription by regulating H2AK119ub

Project description:We found that H2AK119ub could regulate 2 cell genes, Usp17l could also activate 2c gene, and Usp17l could remove ubiquitination modification of H2AK119ub. Therefore, in order to determine whether Usp17l affects 2c gene transcription by regulating H2AK119ub, we knockdown Usp17l. The enrichment of H2AK119ub/H3K27me3 was detected by CUT&Tag . We also sorted out Zscan4 positive and negative cells, and detected the concentration of H3K27me3 by cut-tag

2025-02-19 | GSE224711 | GEO

Effect of Usp17l overexpression on 2c gene in mouse embryonic stem cells

Project description:In order to study the regulation of Usp17l on 2c gene, we overexpressed Usp17le We then performed gene expression profiling with the data obtained from the RNA-seq 48h after Usp17le overexpression .

2023-12-18 | GSE234366 | GEO

Usp17l can regulate the expression of 2C gene through Dux in mouse embryonic stem cells

Project description:In order to study Usp17l can regulate 2C gene expression through Dux, we overexpressed Usp17le and knockdown Dux.Then we performed gene expression profiling with the data obtained from the RNA-seq.

2025-06-05 | GSE277982 | GEO

Effect of Usp17l overexpression on 2c gene in mouse embryonic stem cells

Project description:Effect of Usp17l overexpression on 2c gene in mouse embryonic stem cells

| PRJNA981134 | ENA

USP17L promotes the 2-cell program through deubiquitination of H2AK119ub1 and ZSCAN4 [ChIP-Seq]

Project description:Zygotic genome activation (ZGA) is the first transcription event after fertilization. A minor wave of ZGA (minor ZGA) precedes and is essential for the major wave of ZGA (major ZGA) in two-cell (2C) mouse embryos. A subset of minor ZGA genes (known as “2C” genes) are also activated in a rare population of embryonic stem cells (ESCs) (2C-like cells), which possess expanded developmental plasticity to both embryonic and extra-embryonic tissues, like 2-cell embryos. However, the functions of these 2C genes remain to be fully understood. Here, through knockdown, overexpression, and biochemical analyses, we find that one family of such 2C genes, Usp17l, plays essential roles in both transcriptional and post-translational regulation of the 2C program. Specifically, USP17LE, a member of the USP17L family, deubiquitinates H2AK119ub1 and promotes the expression of Dux and the downstream 2C genes, including Zscan4, Tcstv1, and retrotransposons such as MERVL, in ESCs. Moreover, USP17LE deubiquitinates and stabilizes ZSCAN4. In mouse pre-implantation embryos, Dux is marked by strong H2AK119ub1 at most stages except for the 1-cell and early 2-cell stages, and overexpression of Usp17le reduces H2AK119ub and further promotes the activation of Dux and 2C genes. Thus, our findings identify USP17L as a key regulator of the 2C program.

2025-06-13 | GSE282421 | GEO

USP17L promotes the 2-cell program through deubiquitination of H2AK119ub1 and ZSCAN4 [RNA-Seq]

2025-06-13 | GSE282420 | GEO

Usp17l affects 2c gene transcription by regulating H2AK119ub

Project description:Usp17l affects 2c gene transcription by regulating H2AK119ub

| PRJNA932257 | ENA

Usp17l can regulate the expression of 2C gene through Dux in mouse embryonic stem cells

Project description:Usp17l can regulate the expression of 2C gene through Dux in mouse embryonic stem cells

| PRJNA1165157 | ENA

GADD45 promotes locus specific DNA demethylation and 2C cycling in embryonic stem cells

Project description:Mouse embryonic stem cell cultures contain a rare cell population of "2C-like" cells resembling 2-cell embryos, the key stage of zygotic genome activation (ZGA). Little is known about positive regulators of the 2C-like state and 2-cell stage embryos. We found that GADD45 (growth arrest and DNA damage 45) proteins, regulators of TET mediated DNA demethylation, promote both states. This SuperSeries is composed of the SubSeries listed below.

2019-05-31 | GSE127720 | GEO

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