Project description:Human monocyte-derived dendritic cell cultures devoid of contaminating plasmacytoid dendritic cells were treated with the D-type CpG-oligonucleotide D19, with the non-CpG-oligonucleotide control D, or were left untreated. Cells of all treatment groups stemmed from the same donor. 6 h after addition, cells were harvested, and total RNA was prepared for analysis of D19-dependent differential gene expression on Affymetrix HG-U 133A 2.0. Keywords: agent response
Project description:Human monocyte-derived dendritic cell cultures devoid of contaminating plasmacytoid dendritic cells were treated with the D-type CpG-oligonucleotide D19, with the non-CpG-oligonucleotide control D, or were left untreated. Cells of all treatment groups stemmed from the same donor. 6 h after addition, cells were harvested, and total RNA was prepared for analysis of D19-dependent differential gene expression on Affymetrix HG-U 133A 2.0. Experiment Overall Design: One sample per treatment-group. No replicates.
Project description:Transcription profiling of human monocyte-derived dendritic cells to compare the phenotypes of interferon-alpha and interleukin-4 conditioned dendritic cells
Project description:Transcriptional analysis of primary human monocyte-derived dendritic cells (moDCs) infected with different Zika virus strains and YF17D virus
Project description:This is an ordinary differential equation mathematical model investigating the early responses of human monocyte-derived dendritic cells to infection by two H1N1 influenza A viruses of different clinical outcomes: pandemic A/California/4/2009 and seasonal A/New Caledonia/20/1999.
