Project description:To study heterogenous cellular function and behaviors, we isolated individual cells from freshly resected and dissociated eight human neuroblastoma samples including 2 paired samples of primary tumors and bone marrow metastases, removed the inflammatory infiltrate and DAPI-positive dead cells , and performed single cell analysis using the 10x Genomics Chromium platform.
Project description:Gene expression profile at single cell level of 17 orthotopic-patient derived xenografts from neuroblastoma samples. All xenografts were obtained through the St. Jude Childhood Solid Tumor Network (https://cstn.stjude.cloud/)