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Transcriptional profiles of Wnt/beta-catenin signaling activated cardiomyocytes and the others in the atrioventricular canal of zebrafish heart [RNA-seq]

ABSTRACT: Transcriptional profiles of Wnt/beta-catenin signaling activated cardiomyocytes and the others in the atrioventricular canal of zebrafish heart [RNA-seq]

PROVIDER: PRJNA926356 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23190027.fastq.gz	Fastqsanger.gz
	SRR23190028.fastq.gz	Fastqsanger.gz
	SRR23190029.fastq.gz	Fastqsanger.gz
	SRR23190030.fastq.gz	Fastqsanger.gz
	SRR23190031.fastq.gz	Fastqsanger.gz

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Transcriptional profiles of Wnt/beta-catenin signaling activated cardiomyocytes and the others in the atrioventricular canal of zebrafish heart [RNA-seq]

Project description:To understand the function of Wnt/β-catenin signaling-activated cardiomyocytes (β-cat ON CMs), we performed RNA-Seq on β-cat ON and OFF CMs at the atrioventricular canal (AVC) in the heart, respectively. Our analyses indicated that β-cat ON CMs upregulate genes related to blood vessel development.

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Expression data from embryonic day 15.5 atrioventricular canal regions were isolated from Scx-/- and Scx+/+ mice.

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Bulk RNA-Seq of Wnt signaling activating cardiomyocytes and scRNA-Seq of endothelial cells and atrioventricular canal cells in zebrafish

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Cell type-specific epigenomic analysis in the developing heart

Project description:The molecular mechanisms governing heart development provide an important framework to understand congenital heart disease. The embryonic vertebrate heart tube develops an atrioventricular canal that divides the atrial and ventricular chambers, forms atrioventricular conduction tissue and organizes valve development. To better understand the molecular mechanism underlying atrioventricular canal versus chamber myocardium expression, a double-reporter transgenic mouse line was generated in which the expression of EGFP (green fluorescent protein) and Katushka (red fluorescent protein) are selectively expressed in the atrioventricular canal and in the chamber myocardium, respectively. We assessed the genome-wide H3K27ac pattern in isolated embryonic AV canal and in chamber cardiomyocytes, respectively. EGFP and Katushka-positive cells were purified by FACS and fixed. ChIP was performed with the truemicro ChIP (Diagenode) according to the manufacturer’s protocol using the H3K27ac antibody (abcam ab4729). Chipped DNA were subjected to library preparation using the 5500 Series SOLiD™ Systems ample preparation kit (Applied Biosystems) according to manufactures recommendations and sequenced using the 5500 wildfire system (SOLiD).

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