Project description:This project investigates proteomic adaptations of Bacteroides thetaiotaomicron and Bacteroides acidifaciens in response to hyaluronan as the sole glycosaminoglycan carbon source. Both species were cultivated in a standard medium and in a hyaluronan-based minimal medium, and their proteomes were analyzed by LC-MS/MS. The study aimed to identify global shifts in protein expression between growth conditions and to pinpoint specific proteins involved in hyaluronan degradation pathways. Comparative analysis between the two cultivation conditions provides insight into how gut-associated Bacteroides species regulate metabolic and enzymatic machinery for glycosaminoglycan utilization.
Project description:Purpose: Examining the transcriptome of human gut bacteria (Bacteroides xylanisolvens/Bacteroides ovatus) that grow on mucin O-linked glycans as a sole carbon source Methods: Strains were grown on 10 mg/ml mucin O-linked glycans (MOG) or 5 mg/ml glucose as a sole carbon source in vitro. Fold change was calculated as MOG over glucose. Once cells reached an optical density corresponding to mid-log phase growth, RNA was isolated and rRNA depleted. Samples were multiplexed for sequencing on the Illumina HiSeq platform at the University of Michigan Sequencing Core. Data was analyzed using Arraystar software (DNASTAR, Inc.) Genes with significant up- or down-regulation were determined by the following criteria: genes with an average fold-change >10-fold and biological replicates with a normalized expression level >1% of the overall average RPKM expression level. Results: We identified genes activated in response to mucin O-linked glycans from Bacteroides xylanisolvens/Bacteroides ovatus strains
