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Effect of BFD2 (TGME49_311100) knockout on T. gondii gene expression during BFD1 induction

ABSTRACT: Effect of BFD2 (TGME49_311100) knockout on T. gondii gene expression during BFD1 induction

PROVIDER: PRJNA928724 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23254427_1.fastq.gz	Fastqsanger.gz
	SRR23254427_2.fastq.gz	Fastqsanger.gz
	SRR23254428_1.fastq.gz	Fastqsanger.gz
	SRR23254428_2.fastq.gz	Fastqsanger.gz
	SRR23254429_1.fastq.gz	Fastqsanger.gz

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Effect of BFD2 (TGME49_311100) knockout on T. gondii gene expression during BFD1 induction

Project description:The acute-to-chronic stage transition in Toxoplasma gondii involes a global restructuring of the parasite transcriptome. Prior work identified a single transcription factor (BFD1) that is both necessary and sufficient to establish the chronic-stage program, making it the master regulator of this process. Recently, we found that parasites lacking a CCCH-type zinc finger RNA-binding protein (BFD2) are also unable to produce chronic forms. To understand how BFD2 aids in establishing the BFD1 transcriptional program, we knocked out BFD2 in a BFD1-conditional expression strain, wherein BFD1 is induced by treatment with the small molecule Shield-1.

2023-03-31 | GSE223877 | GEO

Effects of BFD1 (TGME49_200385) and BFD2 (TGME49_311100) deletion on gene expression in Toxoplasma gondii during infection in neurons

Project description:Differentiation of proliferative acute stages into semi-quiescent chronic stages is key for Toxoplasma gondii persistence within an infected host. During natural infection, chronic stages are almost exclusively found in neurons, and it is well appreciated that neurons support high rates of spontaneous differentiation in cell culture. Recently, we found that parasites lacking the RNA-binding protein BFD2 are unable to produce chronic forms, consistent with a block in this develomental program. To compare the effects of BFD1 and BFD2 on chronic-stage transcriptional reprogramming in T. gondii, we profiled the transcriptomes of both 'wildtype' and BFD1- and BFD2-knockout parasites during infection of murine primary neurons.

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Project description:The acute-to-chronic stage transition in Toxoplasma gondii involes a global restructuring of the parasite transcriptome and is induced under alkaline stress. We found that parasites lacking the RNA-binding protein BFD2 are unable to produce chronic forms, consistent with a block in this develomental program. To understand how BFD2 facilitates chronic-stage transcriptional reprogramming in T. gondii, we profiled the transcriptomes of both 'wildtype' and BFD2-knockout parasites under alkaline-stressed and standard culture conditions

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