Project description:To gain a global understanding of the impact of TREM1 silencing, we analyzed the CD45+ tumor infiltrating cells (TICs) of B16F10 tumor-bearing Trem1+/+ and Trem1-/- mice. Utilizing the 10x Genomics Chromium Platform, we analyzed approximately 5390 cells per sample with a coverage rate of 15493 genes per cell.
Project description:Fibrocytes are bone marrow-derived cells expressing fibroblast markers. We used single cell RNA sequencing (scRNA-seq) to analyze the tumor-infiltrating fibrocytes in the lung adenocarcinoma patients
Project description:To investigate how DOPAC suppresses tumors in vivo, we performed single-cell RNA sequencing (scRNA-seq) on CD45+ cells isolated from the tumor tissues of B16-F10-bearing mice.
Project description:Tumor cells and the anatomical location determine the composition and functionality of the non-malignant tumor microenvironment (TME). To determine the influence of the anatomical location, we have compared the transcriptome of CD45+ tumor-infiltrating immune cells isolated from intra- and extra-cranial tumors, respectively. The same tumor cell line was used to generate both tumor models.
Project description:Fibrocytes are bone marrow-derived cells expressing fibroblast markers. We used single cell RNA sequencing (scRNA-seq) to analyze the tumor-infiltrating fibrocytes in the lung adenocarcinoma patients
Project description:We established an organoid-based murine pancreatic cancer model that closely resembles human PDAC. To characterize the tumor-infiltrating immune cell states following combination therapy with immune checkpoint inhibitors (ICIs) targeting PD-1 and CTLA-4, along with agonistic CD40 antibodies (aCD40), we performed RNA sequencing (RNA-seq) on tumor-infiltrating CD45+ cells from our model.