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Transcriptome, Small RNA, and Degradome Sequencing Reveals the Roles of MicroRNAs in Response to High Night Temperature Stress at the Filling Stage of Rice

ABSTRACT: Transcriptome, Small RNA, and Degradome Sequencing Reveals the Roles of MicroRNAs in Response to High Night Temperature Stress at the Filling Stage of Rice

PROVIDER: PRJNA929428 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23269537.fastq.gz	Fastqsanger.gz
	SRR23269538.fastq.gz	Fastqsanger.gz
	SRR23269539.fastq.gz	Fastqsanger.gz
	SRR23269540.fastq.gz	Fastqsanger.gz
	SRR23269541.fastq.gz	Fastqsanger.gz

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Transcriptome, Small RNA, and Degradome Sequencing Reveals the Roles of MicroRNAs in Response to High Night Temperature Stress at the Filling Stage of Rice (degradome)

Project description:we utilized microRNA sequencing to identify differentially expressed miRNAs in rice heat-tolerant line and heat-sensitive line under high night temperature stress .

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Transcriptome, Small RNA, and Degradome Sequencing Reveals the Roles of MicroRNAs in Response to High Night Temperature Stress at the Filling Stage of Rice (degradome)

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Transcriptome, Small RNA, and Degradome Sequencing Reveals the Roles of MicroRNAs in Response to High Night Temperature Stress at the Filling Stage of Rice

Project description:we utilized microRNA sequencing to identify differentially expressed miRNAs in rice heat-tolerant line and heat-sensitive line under high night temperature stress .

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Transcriptome, Small RNA, and Degradome Sequencing Reveals the Roles of MicroRNAs in Response to High Night Temperature Stress at the Filling Stage of Rice

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Transcriptome, Small RNA, and Degradome Sequencing Reveals the Roles of MicroRNAs in Response to High Night Temperature Stress at the Filling Stage of Rice

Project description:Transcriptome, Small RNA, and Degradome Sequencing Reveals the Roles of MicroRNAs in Response to High Night Temperature Stress at the Filling Stage of Rice

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Global gene expression profiles of Oryza sativa wild type Zhonghua11 and mutant gif1 in filling stage

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Project description:To reveal the underlying molecular mechanism of Gif1 action in the control of grain filling and yield improvement, we performed transcriptional profiling of wild type Zhonghua11 and mutant gif1 plants in early filling stage on a global scale using the Affymetrix GeneChip Rice Genome Array Experiment Overall Design: Rice caryopsis were harvested in 7 days after flowering and three biological repeats were performed on Zhonghua11 (wild-type) and gif1 (mutant), respectively.

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Banana sRNAome and degradome identify microRNAs functioning in differential responses to temperature stress

Project description:We report the high-throughput profiling of miRNAs in banana. By deep sequencing and computational and molecular analyses, we identify 113 known and 26 banana-specific miRNAs and we characterize their expression pattern under cold and heat stress. We find that 42 banana miRNAs are temperature-responsive. By degradome sequencing, we identify 60 targets for known miRNAs and half of these targets are regulated by 15 temperature-responsive miRNAs. The correlative expression patterns between several miRNAs and their target genes are further validated via qRT-PCR. Our results provide a foundation for understanding the miRNA-dependent temperature stress response in banana and the characterized correlations between miRNAs and their responses to cold or heat stress could serve as markers in the breeding programs or tools for biotechnological approaches for improving temperature stress tolerance of banana.

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Project description:Favorable grain filling ability is crucial for seed development and plant yield1, with less fertilizer applying is the most urgent goals to meet the growing demands of green and safe food. However, the balance mechanism between grain filling and nutrient elements is still unclear so far. Here, we describe a gene GAF1, specially expressed in endosperm aleurone layer, encoding a phosphate transporter, positively controls rice grain filling and seed development and also contributes to phosphate balance was mapped in our study. To study the regulation pattern of GAF1, we performed the RNA-seq analysis of NIL-GAF1 and NIL-gaf1 at middle grain filling stage in seeds. The data shows that many pathways related to starch and sugar metabolism were enriched, and many starch synthesis related genes and phosphate response genes were up-regulated or down-regulated. These data further support that seed specific expressed GAF1 plays a key role in regulating both phosphate homeostasis and seed development. More importantly, overexpression of GAF1 can significantly improve grain filling and thus yield enhanced plant production in field.

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