RNA Polymerase II, the BAF remodeler and transcription factors synergize to evict nucleosomes
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA930474 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR23310201_1.fastq.gz | Fastqsanger.gz | |||
| SRR23310201_2.fastq.gz | Fastqsanger.gz | |||
| SRR23310202_1.fastq.gz | Fastqsanger.gz | |||
| SRR23310202_2.fastq.gz | Fastqsanger.gz | |||
| SRR23310203_1.fastq.gz | Fastqsanger.gz |
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Project description:Chromatin accessibility is a hallmark of active transcription and entails ATP-dependent nucleosome remodeling, which is carried out by complexes such as Brahma-associated factor (BAF). However, the mechanistic links between transcription, nucleosome remodeling and chromatin accessibility are unclear. Here, we used a chemical-genetic approach coupled with time-resolved chromatin profiling to dissect the interplay between RNA Polymerase II (RNAPII), BAF and DNA-sequence-specific transcription factors in mouse embryonic stem cells. We show that BAF dynamically unwraps and evicts nucleosomes at accessible chromatin regions, while RNAPII promoter-proximal pausing stabilizes BAF chromatin occupancy and enhances ATP-dependent nucleosome eviction by BAF. We find that although RNAPII and BAF dynamically probe both transcriptionally active and Polycomb-repressed genomic regions, pluripotency transcription factor chromatin binding confers locus specificity for productive chromatin remodeling and nucleosome eviction by BAF. Our study suggests a paradigm for how functional synergy between dynamically acting chromatin factors regulates locus-specific nucleosome organization and chromatin accessibility.
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