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Notochord and axial progenitor generation by timely BMP and NODAL inhibition during vertebrate trunk formation (human)

ABSTRACT: Notochord and axial progenitor generation by timely BMP and NODAL inhibition during vertebrate trunk formation (human)

PROVIDER: PRJNA930840 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23322151.fastq.gz	Fastqsanger.gz
	SRR23322152.fastq.gz	Fastqsanger.gz
	SRR23322153.fastq.gz	Fastqsanger.gz
	SRR23322154.fastq.gz	Fastqsanger.gz
	SRR23322155.fastq.gz	Fastqsanger.gz

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Notochord and axial progenitor generation by timely BMP and NODAL inhibition during vertebrate trunk formation

Project description:The formation of the vertebrate body is driven by the progressive and coordinated production of trunk tissues from pools of progenitors located in the posterior of the embryo. Aspects of this process are recapitulated by in vitro models based on pluripotent stem cells (PSCs). However, these models lack several tissue components normally found in the vertebrate trunk. Most strikingly, the notochord, a hallmark of chordates and the source of midline signals that pattern surrounding tissues, is absent from current models of human trunk formation. To investigate how trunk tissue is formed, we performed single-cell transcriptomic analysis of chick embryos. This delineated molecularly discrete progenitor populations, which we spatially locate in the embryo and relate to signalling activity. Guided by this map, we determined how a stereotypical spatial organization of tissue types arises in differentiating human PSCs. This involved LATS1/2 mediated repression of YAP activity facilitating WNT signalling, that, together with FGF mediated ERK1/2 activation, induces the transcription factor Bra/TBXT. In addition, timely inhibition of a WNT-induced NODAL and BMP signalling cascade regulates the proportions of different tissue types produced, including notochordal cells. We exploit this to develop an integrated 3D model of human notochord and neural tissue formation. Together the data provide insight into the mechanisms responsible for the formation of the tissues that comprise the vertebrate trunk and pave the way for future studies of patterning in a tissue-like environment.

2023-03-06 | GSE223189 | GEO

Notochord and axial progenitor generation by timely BMP and NODAL inhibition during vertebrate trunk formation

Project description:Notochord and axial progenitor generation by timely BMP and NODAL inhibition during vertebrate trunk formation

| PRJNA925192 | ENA

Notochord and axial progenitor generation by timely BMP and NODAL inhibition during vertebrate trunk formation (human)

2023-03-06 | GSE224404 | GEO

Notochord and axial progenitor generation by timely BMP and NODAL inhibition during vertebrate trunk formation (human 3D)

2024-02-14 | GSE255338 | GEO

Notochord and axial progenitor generation by timely BMP and NODAL inhibition during vertebrate trunk formation (human 3D)

Project description:Notochord and axial progenitor generation by timely BMP and NODAL inhibition during vertebrate trunk formation (human 3D)

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Axial progenitors generate trunk neural crest cells in vitro

Project description:The in vitro generation of neural crest (NC) cells from human pluripotent stem cells (hPSCs) is a valuable approach to study human NC biology and isolate NC derivatives for disease modelling/regenerative medicine applications. However, conventional differentiation protocols induce only a modest yield of NC cells corresponding to the trunk level. Here we show that trunk NC cells and, their downstream derivatives, sympathoadrenal progenitors, can be produced at a high efficiency from hPSC-derived axial progenitors, the in vitro counterparts of the posteriorly-located drivers of embryonic axis elongation. Moreover, using transcriptome analysis, we define the molecular signatures associated with the emergence of human NC cells of distinct axial identities. Collectively, our findings indicate that a post-cranial NC state is achieved through two different routes: the birth of cardiac and vagal NC is facilitated by retinoic acid-induced posteriorisation of an anterior precursor whereas a trunk fate relies on a posterior axial progenitor intermediate.

2018-08-10 | GSE109267 | GEO

Axial progenitors generate trunk neural crest cells in vitro

Project description:Axial progenitors generate trunk neural crest cells in vitro

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Single cell response landscape of graded Nodal signaling in zebrafish explants [single-cell RNA-seq]

Project description:Purpose: To construct cell landscape of graded Nodal signaling in zebrafish explants. Methods: Nodal injected explants (injected with 10pg ndr2 mRNA) were harvested at 4hpf, 5hpf, 6hpf, 8hpf, 10hpf, 24hpf. Libraries were prepared using Chromium Controller and Chromium Single Cell 3’Library & Gel Bead Kit v2 (10x Genomics, PN-120237) according to the manufacturer’s protocol for 10000 cells recovery. Results: A total of 42,317 single-cell transcriptomes were collected after stringent quality control measures. Conclusions: 19 Cell types from 6 developmental stages were identified, Nodal explant mainly contains the axial mesoderm (prechordal plate and notochord) and anterior-posterior patterned head structures.

2021-01-28 | GSE165653 | GEO

A 37 kb region upstream of Brachyury comprising a notochord enhancer is essential for notochord and tail development

Project description:Genetic dissection of the mouse Brachyury locus identified a notochord enhancer and predicts additional control elements essential for trunk and tail development of the mouse embryo.

2021-11-26 | GSE179665 | GEO

Cdx and Hox genes differentially regulate posterior axial growth in mammalian embryos -13 somites embryonic stage

Project description:Hox and Cdx transcription factors regulate embryonic positional identities. Cdx mutant mice display posterior body truncations of the axial skeleton, neuraxis, and caudal uro-rectal structures. We show that trunk Hox genes stimulate axial extension as they can largely rescue these Cdx mutant phenotypes. Conversely, posterior (paralog group 13) Hox genes can prematurely arrest posterior axial growth when precociously expressed. Our data suggest that the transition from trunk to tail Hox gene expression successively regulates construction and termination of axial structures in the mouse embryo. Thus, Hox genes seem to differentially orchestrate posterior expansion of embryonic tissues during axial morphogenesis as an integral part of their function in specifying head-to-tail identity. In addition, we present evidence that Cdx and Hox transcription factors exert these effects by controlling Wnt signaling. Concomitant regulation of Cyp26a1 expression, restraining retinoic acid signaling in the posterior growth zone, may likewise play a role in timing the trunk-tail transition.

2009-10-19 | GSE17660 | GEO

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