Project description:Cryopreservation of fish embryos is highly challenging, and to date, successful reports of fish embryo cryopreservation remain scarce. In this study, we optimized the vitrification solution and employed gold nanorods (GNRs) in a laser-assisted warming process to improve the hatching rate of Culter alburnus embryos after cryopreservation. With V11 vitrification solution (15% DMSO, 10% EG, 15% PG, and 1 mM MT) and a laser-assisted warming condition (25 μg/mL GNRs with continuous 15 W laser irradiation), we achieved rapid and homogeneous warming, and the mean hatching rate of cryopreserved embryos reached 18.83%. RNA-seq revealed that above cryopreservation procedure led to significant transcriptional changes in calcium signaling pathways and apoptosis-related genes, which may facilitate further optimization of vitrification solution. This study provides a basis for further improving the efficiency of embryo cryopreservation in Culter alburnus and other fish species.
Project description:Chromosome-scale assembly and QTL mapping for major economic traits of Culter alburnus L. genome using Illumina, PacBio sequencing with Hi-C mapping information
