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ABSTRACT: Apple replant disease: a metagenomic approach, eukaryotes

PROVIDER: PRJNA933105 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23422327_1.fastq.gz	Fastqsanger.gz
	SRR23422327_2.fastq.gz	Fastqsanger.gz
	SRR23422328_1.fastq.gz	Fastqsanger.gz
	SRR23422328_2.fastq.gz	Fastqsanger.gz
	SRR23422329_1.fastq.gz	Fastqsanger.gz

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Project description:The normal growth of Nelumbo nucifera, a widely planted aquatic crop in Asia, was severely ruined by replant disease. To investigate changes of transcripts in response to replant disease, transcriptomic analyses were performed by comparing seedings of first-year planting (FP) and consecutive planting (CP). 9810 differentially expressed genes (DEGs) were identified between FP and CP. Based on the biological function of these target genes, the signal pathway of consecutive monoculture was illustrated in N.nucifera. The experiments aimed to provide data for unveiling the molecular characteristics of replant disease, and explore our understanding of replant disease in aquatic botony.

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Real-time quantitative PCR analysis of apple roots and leaves after ARD infection

Project description:Root and leave samples of 4 different apple genotypes were investigated in order to analyse the gene expression after infection with Apple Replant Disease (ARD). All genotypes were cultivated in ARD-infected soil and gamma-irradiated (disinfected) soil in the greenhouse for 7 days. The ARD soil originated from two different orchards representing two different soil compositions. After 7 days root tissue was collected from each plant and used for the subsequent gene expression analysis. This work was part of the project BonaRes-ORDIAmur funded by the German Federal Ministry of Research and Education within the frame of the program BonaRes (grant no. 031B0025B). It was also funded by the German Research Foundation (DFG) via the research training group GRK1798 "Signaling at the Plant-Soil Interface" and a grant to BL and LB (BE 1174/19-1).

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