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ABSTRACT: Copper ion mediates yeast-to-hypha transition in Yarrowia lipolytica

PROVIDER: PRJNA933354 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23388412.fastq.gz	Fastqsanger.gz
	SRR23388413.fastq.gz	Fastqsanger.gz
	SRR23388414.fastq.gz	Fastqsanger.gz
	SRR23388415.fastq.gz	Fastqsanger.gz
	SRR23388416.fastq.gz	Fastqsanger.gz

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Project description:The yeast Yarrowia lipolytica Y-2 shown the capability to degrade OTA by the intracellular enzymes. However, the enzymes which risponsible for the degradation process was unkonown. Transcriptome change in response to mycotoxin OTA was analyzed. The molecular mechanism of Yarrowia lipolytica Y-2 withstand OTA was revealed.

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Project description:Total RNA versus genomic DNA hybridization on custom arrays designed for all Yarrowia lipolytica genes Total RNA was collected in mid-log phase from Yarrowia lipolytica cells grown in rich medium (abbreviated CM, in house recipe). RNA was then converted to cDNA, Cy3-labeled and hybridized competitively against Cy5 labeled genomic DNA from Yarrowia lipolytica.

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