Project description:Previous studies have evaluated pork quality by omics methods. However, proteomics coupled with metabolomics to investigate pork freshness by using pork exudates has not been reported. This study determined the changes in profiles of peptides and metabolites in exudates from pork stored at different temperatures (25, 10, 4, and -2 ℃). Multivariate statistical analysis revealed similar changes in profiles in exudates collected from pork stored at -2 and 4 ℃, and additional changes following storage at higher temperatures. We identified peptides from 7 proteins and 30 metabolites differing in abundance between fresh and spoiled pork. Significant correlations be-tween pork quality and most of the peptides from these 7 proteins and 30 metabolites were found. The present study provides insight into changes in peptide and metabolite profiles of exudates from pork during storage at different temperatures and our analysis suggest that such changes can be used as markers for pork spoilage.
Project description:Intramuscular (i.m.) fat content influencing consumer’s acceptability of pork is considered as a limiting factor for meat quality. To gain insight into the biological basis of individual variability in i.m. fat content, both gene expression profiling and proteomic investigation were associated in pig longissimus muscle (LM). Keywords: intramuscular fat, gene expression, pigs, proteomics, microarray, pork meat
Project description:To understand the molecular basis of distinct pork quality in Chinese indigenous and Western breed, longissimus dorsi samples were collected from three adult Northeastern Indigenous and from three adult Large White. Total RNA was extracted and subjected to porcine Affymetrix Genechip. The study helps to elucidate the genetic mechnism of divergent pork quality and provide the theory basis for selection and genetic improvement of meat quality traits in porcine.
Project description:Intramuscular (i.m.) fat content influencing consumerâs acceptability of pork is considered as a limiting factor for meat quality. To gain insight into the biological basis of individual variability in i.m. fat content, both gene expression profiling and proteomic investigation were associated in pig longissimus muscle (LM). Keywords: intramuscular fat, gene expression, pigs, proteomics, microarray, pork meat Animals were sampled from a population of 1,000 pigs generated as an F2 intercross between two production sire lines: FH016 (Pietrain type, France Hybrides SA, St Jean de Braye, France) and FH019 (Synthetic line, from Duroc, Hampshire and Large White founders, France Hybrides SA, St Jean de Braye, France).
Project description:In this study, pork intramuscular fat (IMF) content and tenderness, myosin heavy chain (MYH) isoform expression was characterized in four cuts including longissimus dorsi muscle (LDM), trapezius muscle (TM), hamstring muscle (HM) and pork belly (PB). Then transcription expression profiles were compared in LDM, TM, HM and PB of Chulu black pig using RNA sequencing technologies (RNA-Seq). We profiled the transcriptome of HM, LDM, PB and TM isolated from pigs with approximately 100kg body weight and identified expressed 21280 transcription units. Of the them, 292-1589 DEGs genes were differentially expressed between four cuts. Using analysis of upset Venn graph, the 47, 305, 395 and 10 unique DEGs were respectively screened for HM, LDM, PB and TM. The enriched pathways in unique DEGs of HM mainly related to “organismal systems and inflammatory mediator regulation of TRP channels”, “neurotrophins signaling pathway” and “cellular senescence”. In unique DEGs of LDM, pathways included “glycolysis / Gluconeogenesis”, “carbon metabolism”, “HIF-1 signaling pathway”, “glucagon signaling pathway”, “pentose phosphate pathway” and “PPAR signaling pathway” were significantly enriched. In PB, the specific DEGs enriched in the human disease, cellular process and organismal systems pathways. Only two significantly enriched pathways including “inflammatory mediator regulation of TRP channels” and “axon guidance” were found in unique DEGs of TM. The findings suggested that the cuts differences of the pork are not only related to amino acid metabolism and lipid metabolism, but also closely associated with muscle fibers type and the formation of flavor precursor substances. The cut specific gene expression patterns finally decided the VOCs profile in pork constituting the unique aroma of each meat cuts of ChaLu black pork.
Project description:To understand the molecular basis of distinct pork quality in Chinese indigenous and Western breed, longissimus dorsi samples were collected from three adult Northeastern Indigenous and from three adult Large White. Total RNA was extracted and subjected to porcine Affymetrix Genechip. The study helps to elucidate the genetic mechnism of divergent pork quality and provide the theory basis for selection and genetic improvement of meat quality traits in porcine. Six longissimus dorsi samples were collected from three Northeastern Indigenous and from three Large White. Three Large White were control samples. Total RNA was extracted from each sample.Gene-expression profiling was performed for each RNA sample separately on the GeneChip® Porcine Genome Array at CapitalBio Corporation (Beijing, China).
Project description:Transcriptional profiling of two disinfectant-resistant Listeria monocytogenes strains indentified in a Iberian pork plant, S1 [160908] vs S10_1 [160908], in presence of 1.25 mg/L of Benzalkonium chloride