Project description:Purpose: We wanted to explore further heterogeneity of MBC subsets that were previously defined by our lab. Methods: MBC subsets were sorted and stained with unique anti-mouse HTO antibodies (CITE-seq). Results: We found that heterogeneity among MBC subsets was most prominent among DP MBC subsets, which could be separated by extrafollicular or germinal center transcriptional signatures, whereas DN and SP MBCs mostly exhibited an extrafollicular signature.
Project description:To understand the transcriptional circuits controled by the histone methyltransferase EZH2 in memory B cells (MBC) scRNA-seq was performed on influenza-specific MBC that formed at an early day 14 or late day 39 time point. EZH2-knockout MBC were compared to CRE control MBC isolated from the spleens and mediastinal lymph nodes of mice.