Ontology highlight
ABSTRACT:
PROVIDER: PRJNA937385 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Phytoplankton and bacteria form the base of marine ecosystems and their interactions drive global biogeochemical cycles. The effect of bacteria and bacteria-produced compounds on diatoms range from synergistic to pathogenic and can affect the physiology and transcriptional patterns of the interacting diatom. Here, we investigate physiological and transcriptional changes in the marine diatom Thalassiosira pseudonana induced by extracellular metabolites of a known antagonistic bacterium Croceibacter atlanticus. Mono-cultures of C. atlanticus released compounds that inhibited diatom cell division and elicited a distinctive phenotype of enlarged cells with multiple plastids and nuclei, similar to what was observed when the diatom was co-cultured with the live bacteria. The extracellular C. atlanticus metabolites induced transcriptional changes in diatom pathways that include recognition and signaling pathways, cell cycle regulation, carbohydrate and amino acid production, as well as cell wall stability. Phenotypic analysis showed a disruption in the diatom cell cycle progression and an increase in both intra- and extracellular carbohydrates in diatom cultures after bacterial exudate treatment. The transcriptional changes and corresponding phenotypes suggest that extracellular bacterial metabolites, produced independently of direct bacterial-diatom interaction, may modulate diatom metabolism in ways that support bacterial growth.
2022-06-24 | GSE197934 | GEO
Project description:We isolate the cultivable microbiome of a diatom and show that different bacteria have commensal, antagonistic, or synergistic effects on the diatom. One synergistic bacterium enhances growth of the diatom by production of auxin, a phytohormone. The diatom and its synergistic bacterium appear to use auxin and tryptophan as signaling molecules that drive nutrient exchange. Detection of auxin molecules and biosynthesis gene transcripts in the Pacific Ocean suggests that these interactions are widespread in marine ecosystems.
2015-05-29 | GSE65189 | GEO
Project description:Phytoplankton-bacteria interactions are pivotal in marine ecosystems, influencing primary production and biogeochemical cycles. Diatoms engage in diverse relationships with bacteria, ranging from mutualism to pathogenicity. This study explores the interaction between a novel Alteromonas macleodii strain from the Equatorial Pacific and the model Thalassiosira pseudonana across the diatom different growth phases. We demonstrate that A. macleodii’s algicidal activity depends on the diatom’s growth phase, defensive capacity, and nutrient availability. The algicidal effect manifests during the diatom’s stationary phase or with external nutrient supplementation, implicating organic matter availability as a key driver. Transcriptomic analysis reveals that A. macleodii shifts from motility-associated to growth-associated gene expression based on the diatom’s physiology and coculture duration. Filtrate assays and fluorescence microscopy suggest a two-stage infection model: initial bacterial motility and exudate secretion induce diatom death, followed by bacterial aggregation around debris. Comparative transcriptomics with other algal hosts highlights host-specific bacterial responses, underscoring the context-dependent nature of these interactions. Our findings provide a deeper understanding of the molecular mechanisms driving diatom-bacteria interactions, shedding light on their role in marine microbial ecology and ecosystem functioning.
2026-02-10 | GSE291849 | GEO
Project description:Diatom-associated bacteria Genome sequencing and assembly
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Project description:Population genomics of macroalgal associated epifauna
| PRJNA770423 | ENA
Project description:diatom-bacteria co-cultures, axenic diatom and bacteria controls 16S rDNA Raw sequence reads
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Project description:Diatom associated community Metagenomic assembly
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