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ABSTRACT: Ophiocomina nigra ddRAD data

PROVIDER: PRJNA938024 | ENA |

REPOSITORIES: ENA

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Dataset's files

Source:

			Action	DRS
	SRR23597500.fastq.gz	Fastqsanger.gz
	SRR23597501.fastq.gz	Fastqsanger.gz
	SRR23597502.fastq.gz	Fastqsanger.gz
	SRR23597503.fastq.gz	Fastqsanger.gz
	SRR23597504.fastq.gz	Fastqsanger.gz

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Spiked human substantia nigra proteome data set for use as a spectral library for protein modelling and protein mapping

Project description:This article describes a mass spectrometry data set generated from human substantia nigra tissue that was spiked with iRT peptides. The data set can be used as a spectral library for analysis of the human brain, especially human substantia nigra, for example, in the context of Parkinson’s disease. Obtaining a sufficient amount of high-quality substantia nigra tissue is the key limiting factor for establishing a brain region-specific spectral library. Hence, combining existing spectral libraries for data-independent acquisition analysis (DIA) can overcome this major limitation. Moreover, these data can be used to map brain region-specific proteins and to model brain region-specific pathways. Both can improve our understanding of the functioning of the brain in greater depth. In addition, these data can also be used to determine the optimal settings for measuring proteins and peptides of interest. To create the substantia nigra-specific spectral library, the tissue was first homogenized and then fractionated via different types of SDS gel electrophoresis, resulting in 18 fractions. These fractions were analysed in triplicate by nanoHPLC-ESI-MS/MS, resulting in 54 data files.

2019-08-15 | PXD011076 | Pride

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