Project description:Male Sprague-Dawley albino rats (Charles River) initially weighting approximately 200 grams were randomly assigned to one of the following: 1) olanzapine (2 mg/kg/day, I.P.) (N=20) or 2) Saline (N=20) and administered olanzapine or saline for 21 days. Frontal cortex (N=4/group) was dissected and homogenized for microarray, QPCR and western blotting following previous methods (Fatemi et al. 2003; Brooks et al. 2003). RNA isolation, cDNA preparation, microarray hybridization and QPCR followed previous methods (Brooks et al. 2003).
Project description:Purpose: Sequencing analysis of plasma cell-free RNA was performed to study the effects of space flight and microgravity environment in mice. Methods: Plasma samples were collected during the JAXA MHU-1 mission as reported previously (Shiba et al., 2017, Scientific Reports). Total RNA was purified from plasma samples and processed for sequencing analysis, to compare RNA expression profiles in ground control, space flight and artificial 1-G control groups.
Project description:Inflammation is a key component of pathological angiogenesis. Here we induce cornea neovascularisation using sutures placed into the cornea, and sutures are removed to induce a regression phase. We used whole transcriptome microarray to monitor gene expression profies of several genes
