Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: E. coli plasmid harbouring colistin resistance gene

PROVIDER: PRJNA939939 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other
	SRR23651722_1.fastq.gz	Fastqsanger.gz
	SRR23651723_1.fastq.gz	Fastqsanger.gz
	SRR23651723_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 4 of 4

Similar Datasets

A Noncanonical Intrinsic Terminator in the HicAB Toxin‒Antitoxin Operon Promotes the Transmission of Conjugative Antibiotic Resistance Plasmids

Project description:Conjugative plasmids, major vehicles for the spread of antibiotic resistance genes, often contain multiple toxin‒antitoxin (TA) systems. However, the physiological functions of TA systems remain obscure. By studying TA families commonly found on colistin-resistant IncI2 mcr-1-bearing plasmids, we discovered that the HicAB TA, acts as a crucial addiction module to increase horizontal plasmid‒plasmid competition.

2024-12-25 | GSE284789 | GEO

Escherichia coli

Project description:Plasmid sequencing of tetracycline resistance genes harbouring plasmid.

| PRJDB11322 | ENA

Escherichia coli

Project description:Plasmid sequencing of tetracycline resistance genes harbouring plasmid.

| PRJDB11334 | ENA

Escherichia coli

Project description:Plasmid sequencing of tetracycline resistance genes harbouring plasmid.

| PRJDB11335 | ENA

Plasmid mediated mcr-1.1 colistin-resistance in clinical extraintestinal Escherichia coli strains isolated in Poland

Project description:Plasmid mediated colistin-resistance in E. coli isolated in Poland

| PRJEB42972 | ENA

Transcriptomic analysis of paired patient isolates of Acinetobacter baumannii following failed colistin treatment

Project description:Colistin is a crucial last-line drug used for the treatment of life-threatening infections caused by multi-drug resistant strains of the Gram-negative bacteria, Acinetobacter baumannii. However, colistin resistant A. baumannii isolates can be isolated following failed colistin therapy. Resistance is most often mediated by the addition of phosphoethanolamine (pEtN) to lipid A by PmrC, following missense mutations in the pmrCAB operon encoding PmrC and the two-component signal transduction system PmrA/PmrB. We recovered an isogenic pair of A. baumannii isolates from a single patient before (6009-1) and after (6009-2) failed colistin treatment that displayed low/intermediate and high levels of colistin resistance, respectively. To understand how increased colistin-resistance arose, we genome sequenced each isolate which revealed that 6009-2 had an extra copy of the insertion sequence element ISAba125 within a gene encoding an H-NS-family transcriptional regulator. Consequently, transcriptomic analysis of the clinical isolates identified was performed and more than 150 genes as differentially expressed in the colistin-resistant, hns mutant, 6009-2. Importantly, the expression of eptA, encoding a second lipid A-specific pEtN transferase, but not pmrC, was significantly increased in the hns mutant. This is the first time an H-NS-family transcriptional regulator has been associated with a pEtN transferase and colistin resistance.

2018-02-19 | GSE107964 | GEO

RNAseq by using Illumina Hiseq (RNA library was generated using TruSeq RNA library prep kit)

Project description:Acinetobacter baumannii is often highly resistant to multiple antimicrobials, posing a risk of treatment failure. Colistin is often chosen as a “last resort” for treatment of the bacterial infection, but resistance is easily developed when the bacteria is exposed to the drug. Thus a comprehensive analysis of colistin-mediated changes in colistin-susceptible and colistin-resistant A. baumannii is needed. In this study, we used a colistin-susceptible A. baumannii clinical isolate and a colistin-resistant isogenic mutant. Whole genome sequencing revealed that the resistant isolate harbored a PmrBL208F mutation conferring colistin resistance, and all other single nucleotide alterations were located in intergenic regions. Using scanning electron microscopy, we observed that the colistin-resistant mutant had a shorter cell length than the parental isolate, and filamented cells were observed when both isolates were exposed to inhibitory concentration of colistin. When the isolates were treated with inhibitory concentrations of colistin, more than 80% of the genes were upregulated, including genes associated with antioxidative stress response pathways. This results helped a better understanding for the morphological difference between the colistin-susceptible and –resistant isolates and differed colistin-mediated responses in A. baumannii isolates by their susceptibility to this drug.

2025-05-02 | GSE267612 | GEO

Project description:Plasmid mediated mcr-1.1 colistin-resistance in clinical extraintestinal Escherichia coli strains isolated in Poland

| PRJNA700422 | ENA

mcr-3

Project description:Plasmid-borne colistin resistance gene mcr-3 in Salmonella isolates from human infections.

| PRJEB23266 | ENA

Emergence of a clonal lineage of multidrug-resistant extended spectrum beta-lactamase (ESBL)-producing Salmonella Infantis transmitted from broilers and broiler meat to humans in Italy between 2011 and 2014

Project description:To demonstrate plasmid transferability by conjugation, cultures of the donor S. Infantis, and recipient Escherichia coli (E. coli) K12 were mated. S. Infantis and transconjugant were screened for resistance genes.

2016-01-12 | E-MTAB-4108 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data