Ontology highlight
ABSTRACT:
PROVIDER: PRJNA940147 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR23675102_1.fastq.gz | Fastqsanger.gz | |||
| SRR23675102_2.fastq.gz | Fastqsanger.gz | |||
| SRR23675103_subreads.fastq.gz | Fastqsanger.gz | |||
| SRR23675104_subreads.fastq.gz | Fastqsanger.gz | |||
| SRR23675105_subreads.fastq.gz | Fastqsanger.gz |
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Project description:Quinoa (Chenopodium quinoa) is a highly nutritious crop showing a remarkable tolerance to multiple abiotic stresses. The Catharanthus roseus RLK1-like kinase (CrRLK1L) family is involved in multiple processes during plant growth and stress responses. However, little is known about the members of the CqCrRLK1L subfamily and their biological functions. In this study, up to 26 CqCrRLK1L members were identified in quinoa genome. To systematically investigate their tissue-specific expression pattern and gene expression profiles in response to abiotic stresses, different quinoa samples including leaf, pistil, stamen, root, and leaf treated with NaCl were harvested for transcrioptome analysis with three biological replicates for each sample. Finally,we mapped about 30 million sequence reads per sample to the quinoa genome (NL-6) and combining the four different tissues (leaf, pistil, stamen, and root), totally 40382 genes were detected, accounting for 69% of total genes in quinoa. The expression of most CqCrRLK1Ls genes were detected in all four kind of tissues, and they showed tissue-specific expression pattern. In salt-treated leaves, we observe that about 6-thosands genes changed their expression, with log2FoldChange < -1 or >1 and p value <0.05. And four CqCrRLK1L genes largely altered their transcript levels, implying that these genes are involved in the regulation of salt stress response in quinoa. The present study provides a base for future research on elucidating the varied biological functions of CqCrRLK1Ls and their contributions to stress responses.
2022-06-27 | GSE198572 | GEO
Project description:We generated 95.37 Gb of high-quality sequencing data (~7.95 Gb per sample). The analysis showed differences of transcriptomes between the common white sweet quinoa and the yellow bitter quinoa. We identified numerous differentially expressed genes that exhibited distinct expression patterns. These genes have known or potential roles in taste of quinoa fruit.Therefore, we are appealing candidates for further investigation of the gene expression and associated regulatory mechanisms related to the accumulation of bitter saponins in C. quinoa fruits.
2021-09-17 | GSE139174 | GEO
Project description:We generated 70.9 Gb of high-quality sequencing data (~7.88 Gb per sample) and catalogued the expression profiles of 54，238 annotated Chenopodium quinoa genes in each sample. These genes have known or potential roles in the roots, stems, and leaves of quinoa. Therefore, we are appealing candidates for further investigation of the gene expression and associated regulatory mechanisms.
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Project description:Quinoa is a highly nutritious seudocereal crop that is not well adapted to heat. Improving understanding of quinoa responses to heat would help expand its cultivation to regions with warmer climates. This study profiled gene expression of quinoa plants from accession QQ74 (PI 614886) treated with high tempreatures during anthesis of the main panicle, which lasted 11 days. Heat was applied in four different treatments to profile gene expression changes from root or shoot heating. Treatments were: 1. control with both roots and shoots at 22°C, 2. heated roots with roots at 30°C and shoots at 22°C, 3. heated shoots with roots at 22 °C and shoots at 35°C, and 4. heated roots and shoots with roots at 30°C and shoots at 35°C. Healthy leaves from 3 plants per treatment were sampled from the mid section of the plants, during the first (day 1) and last day (11) of heat treatment, at Zeitgeber time 2. Gene expression of each of the three heat treatments was compared to control using the Kallisto-Sleuth pipeline. These gene expression profiles of quinoa under heat are made available as a community resource to improve understanding of quinoa responses to heat.
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Project description:<p>Quinoa (<em>Chenopodium quinoa</em> Willd.) is an herbaceous annual crop of the amaranth family (Amaranthaceae). It is increasingly cultivated for its nutritious grains, which are rich in protein and essential amino acids, lipids, and minerals. Quinoa exhibits a high tolerance towards various abiotic stresses including drought and salinity, which supports its agricultural cultivation under climate change conditions. The use of quinoa grains is compromised by anti-nutritional saponins, a terpenoid class of secondary metabolites deposited in the seed coat; their removal before consumption requires extensive washing, an economically and environmentally unfavorable process; or their accumulation can be reduced through breeding. In this study, we analyzed the seed metabolomes, including amino acids, fatty acids, and saponins, from 471 quinoa cultivars, including two related species, by liquid chromatography - mass spectrometry. Additionally, we determined a large number of agronomic traits including biomass, flowering time, and seed yield. The results revealed considerable diversity between genotypes and provide a knowledge base for future breeding or genome editing of quinoa.</p>
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2018-09-20 | E-MTAB-6112 | biostudies-arrayexpress