Project description:In this work, we describe the transcriptional profiles of Baikal omul juveniles after acute and chronic temperature stress exposure. The juveniles were kept for 1.5 months at 9–12 °C, followed by exposure to acute stress (heating to 21 °C for 1 hour) and chronic stress (heating to 21 °C for 24 hours 3 times a week for a month) in the Experimental Freshwater Aquarium Complex for Baikal Hydrobionts at the Limnological Institute (LIN SB RAS). The information on the transcriptional profiles will contribute to further understanding of the mechanisms of adaptation of whitefish to the environment.
Project description:In this study, the transcriptional profiles of four deep-sea sculpin fish species (Cottoidei) are described for the first time as part of the study of the adaptation of endemic fishes to different habitats in Lake Baikal. These studies will lead to a deeper understanding of the molecular mechanisms that ensure the adaptation of endemic species to specific habitats in Lake Baikal, even at depths of more than 500 m (up to 1600 m).
Project description:In this work, we describe the transcriptional profiles of adapted and non-adapted one-month-old Baikal whitefish juveniles after heat shock exposure. Preadapted fish were exposed to a repeated thermal rise of 6 °C above control temperature every 3 days throughout embryonic development. One month after hatching, preadapted and non-adapted larvae were either maintained at control temperatures (12 °C) or exposed to an acute thermal stress (TS) of 12 °C above control temperature. The information on transcriptional profiles will contribute to further understanding of the mechanisms of adaptation of whitefish to the environment.
Project description:Hemocytes are blood cell circulating in the hemolymph and playing important role in crustacean immunity. On the one side this cells functions as phagocytes and express immune compounds in the external milieu on the other side. To find out the protein composition of hemocytes, we obtained hemocytes proteome of Baikal endemic amphipod Eulimnogammarus verrucosus for the first time. For this, a proteomic analysis of the protein extract were performed using liquid chromatography/tandem mass spectrometry (LC-MS/MS). A total of 1152 unique proteins were discovered. Hemocyanin/phenoloxidase, actin and tubulin were detected in proteome in the greatest amount. Also we discovered proteins of the C-type lectin superfamily, which are probably involved in the molecular pattern recognition during immune response.