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Different immunological mechanisms between AQP4-positive and MOG-positive optic neuritis based on RNA-seq analysis of whole blood

ABSTRACT: Different immunological mechanisms between AQP4-positive and MOG-positive optic neuritis based on RNA-seq analysis of whole blood

PROVIDER: PRJNA941826 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23719920_1.fastq.gz	Fastqsanger.gz
	SRR23719920_2.fastq.gz	Fastqsanger.gz
	SRR23719921_1.fastq.gz	Fastqsanger.gz
	SRR23719921_2.fastq.gz	Fastqsanger.gz
	SRR23719922_1.fastq.gz	Fastqsanger.gz

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Different immunological mechanisms between AQP4-positive and MOG-positive optic neuritis based on RNA-seq analysis of whole blood

Project description:To compare the different immunological mechanisms between aquaporin 4 antibody-associated optic neuritis (AQP4-ON) and myelin oligodendrocyte glycoprotein antibody-associated optic neuritis (MOG-ON) based on RNA sequencing (RNA-seq) of whole blood.

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PBMC gene expression of early onset of the first demyelinating event of acute optic neuritis

Project description:Optic neuritis (ON) is a common manifestation of multiple sclerosis (MS); it appears as the presenting symptom in about 25% of MS patients and occurs in 30–70% of patients with MS during the course of their illness Purpose. To evaluate the molecular pathways that operate in the early phase of acute ON by studying gene expression profiles of peripheral blood mononuclear cells (PBMCs) subpopulations including CD19+ B cells, CD14+ macrophages, CD4+ and CD8+ T cells. High throughput gene expression analysis was performed on periferal mononuclear blood cells (PBMC) samples from 6 patients within 96 hours of the acute onset of the first demyelinating event of optic neuritis and 9 age matched healthy subjects using Affymetrix Inc. technology

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Transcriptome Sequencing of Optic Nerve in a Rat Model of Neuromyelitis Optica Spectrum Disorder-Related Optic Neuritis (NMOSD-ON)

Project description:This study established a rat model of NMOSD-ON by injecting patient-derived AQP4+ serum into the optic nerve sheath of rats. Optic nerve tissues were collected at 24 hours and 7 days post-modeling, along with a blank control group, for transcriptome sequencing. The aim was to explore gene expression changes during the early and peak phases of NMOSD-ON pathogenesis, thereby identifying potential disease mechanisms.

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MOG-expressing teratoma followed by MOG-IgG-positive optic neuritis.

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Different immunological mechanisms between AQP4 antibody-positive and MOG antibody-positive optic neuritis based on RNA sequencing analysis of whole blood.

Project description:PurposeTo compare the different immunological mechanisms between aquaporin 4 antibody-associated optic neuritis (AQP4-ON) and myelin oligodendrocyte glycoprotein antibody-associated optic neuritis (MOG-ON) based on RNA sequencing (RNA-seq) of whole blood.MethodsWhole blood was collected from seven healthy volunteers, 6 patients with AQP4-ON and 8 patients with MOG-ON, and used for RNA-seq analysis. An examination of immune cell infiltration was performed using the CIBERSORTx algorithm to identify infiltrated immune cells.ResultsRNA-seq analysis showed that the inflammatory signaling was mainly activated by TLR2, TLR5, TLR8 and TLR10 in AQP4-ON patients, while which was mainly activated by TLR1, TLR2, TLR4, TLR5 and TLR8 in MOG-ON patients. Biological function identification of differentially expressed genes (DEGs) based on Gene Ontology (GO) term and Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis, as well as Disease Ontology (DO) analysis, showed that the inflammation in AQP4-ON was likely mediated by damage-associated molecular pattern (DAMP), while which in MOG-ON was likely mediated by pathogen-associated molecular pattern (PAMP). Analysis of immune cell infiltration showed that the proportion of immune cell infiltration was related to patients' vision. The infiltration ratios of monocytes (rs=0.69, P=0.006) and M0 macrophages (rs=0.66, P=0.01) were positively correlated with the BCVA (LogMAR), and the infiltration ratio of neutrophils was negatively correlated with the BCVA (LogMAR) (rs=0.65, P=0.01).ConclusionThis study reveals different immunological mechanisms between AQP4-ON and MOG-ON based on transcriptomics analysis of patients' whole blood, which may expand the current knowledge regarding optic neuritis.

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The proteome of human retrobulbar optic nerve

Project description:The optic nerve is a white matter tract that conveys visual information to the brain. A detailed investigation of the proteome of the normal human retrobulbar optic nerve may help facilitate studies of the biology and pathophysiology of the optic nerve. We conducted an in-depth proteomic analysis of optic nerve from five adults. Proteins were fractionated using SDS-PAGE. After in-gel digestion, peptides were analyzed using LC-MS/MS on an Orbitrap Elite mass spectrometer. We identified 2,711 non-redundant proteins in the human retrobulbar optic nerve, including the astrocytic marker glial fibrillary acidic protein, several proteins expressed by oligodendrocytes (laminin, proteolipid protein, and fibronectin), myelin proteins (myelin basic protein, myelin-associated glycoprotein), paranodal structural proteins (neurofascin, contactin, ?, ?, and ? adducins, septin 2, endophilin, ankyrin ?, spectrin), proteins involved in neuronal protection and regeneration (? crytallins A and B, dedicator of cytokinesis proteins, ciliary neurotrophic factor), proteins associated with open-angle glaucoma (thioredoxin, heat shock protein-70), and proteins associated with optic neuritis (aquaporin-4). Twenty-one unambiguous protein isoforms were identified in the optic nerve.

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