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A Drosophila screen identifies a role for histone methylation in ER stress preconditioning

ABSTRACT: A Drosophila screen identifies a role for histone methylation in ER stress preconditioning

PROVIDER: PRJNA942314 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23752455_1.fastq.gz	Fastqsanger.gz
	SRR23752455_2.fastq.gz	Fastqsanger.gz
	SRR23752456_1.fastq.gz	Fastqsanger.gz
	SRR23752456_2.fastq.gz	Fastqsanger.gz
	SRR23752457_1.fastq.gz	Fastqsanger.gz

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A Drosophila screen identifies a role for histone methylation in ER stress preconditioning

2023-04-25 | GSE226958 | GEO

A Drosophila screen identifies a role for histone methylation in ER stress preconditioning.

Project description:Stress preconditioning occurs when transient, sublethal stress events impact an organism's ability to counter future stresses. Although preconditioning effects are often noted in the literature, very little is known about the underlying mechanisms. To model preconditioning, we exposed a panel of genetically diverse Drosophila melanogaster to a sublethal heat shock and measured how well the flies survived subsequent exposure to endoplasmic reticulum (ER) stress. The impact of preconditioning varied with genetic background, ranging from dying half as fast to 4 and a half times faster with preconditioning compared to no preconditioning. Subsequent association and transcriptional analyses revealed that histone methylation, and transcriptional regulation are both candidate preconditioning modifier pathways. Strikingly, almost all subunits (7/8) in the Set1/COMPASS complex were identified as candidate modifiers of preconditioning. Functional analysis of Set1 knockdown flies demonstrated that loss of Set1 led to the transcriptional dysregulation of canonical ER stress genes during preconditioning. Based on these analyses, we propose a preconditioning model in which Set1 helps to establish an interim transcriptional "memory" of previous stress events, resulting in a preconditioned response to subsequent stress.

| S-EPMC11021027 | biostudies-literature

A <i>Drosophila</i> screen identifies a role for histone methylation in ER stress preconditioning.

Project description:Stress preconditioning occurs when transient, sublethal stress events impact an organism's ability to counter future stresses. Although preconditioning effects are often noted in the literature, very little is known about the underlying mechanisms. To model preconditioning, we exposed a panel of genetically diverse Drosophila melanogaster to a sublethal heat shock and measured how well the flies survived subsequent exposure to endoplasmic reticulum (ER) stress. The impact of preconditioning varied with genetic background, ranging from dying half as fast to four and a half times faster with preconditioning compared to no preconditioning. Subsequent association and transcriptional analyses revealed that histone methylation, transcriptional regulation, and immune status are all candidate preconditioning modifier pathways. Strikingly, almost all subunits (7/8) in the Set1/COMPASS complex were identified as candidate modifiers of preconditioning. Functional analysis of Set1 knockdown flies demonstrated that loss of Set1 led to the transcriptional dysregulation of canonical ER stress genes during preconditioning. Based on these analyses, we propose a model of preconditioning in which Set1 helps to establish an interim transcriptional 'memory' of previous stress events, resulting in a preconditioned response to subsequent stress.

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A human tissue screen identifies a regulator of ER secretion as a brain size determinant [organoid - screen]

Project description:A human tissue screen identifies a regulator of ER secretion as a brain size determinant [organoid - screen]

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A human tissue screen identifies a regulator of ER secretion as a brain size determinant

Project description:A human tissue screen identifies a regulator of ER secretion as a brain size determinant. Abstract: Loss-of-function (LOF) screens provide a powerful approach to identify regulators in biological processes. Pioneered in laboratory animals, LOF screens of human genes are currently restricted to two-dimensional (2D) cell culture hindering testing of gene functions requiring tissue context. Here we present CRISPR-LIneage tracing at Cellular resolution in Heterogenous Tissue (CRISPR-LICHT), enabling parallel LOF studies in human cerebral organoid tissue. We used CRISPR-LICHT to test 173 microcephaly candidate genes revealing 25 to be involved in known and uncharacterized microcephaly-associated pathways. We characterized Immediate Early Response 3 Interacting Protein 1 (IER3IP1) regulating the unfolded protein response (UPR) and extracellular matrix (ECM) protein secretion crucial for tissue integrity, with dysregulation resulting in microcephaly. Our human tissue screening technology identifies microcephaly genes and mechanisms involved in brain size control.

2021-11-10 | PXD019350 | Pride

A human tissue screen identifies a regulator of ER secretion as a brain size determinant

Project description:A human tissue screen identifies a regulator of ER secretion as a brain size determinant

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Analysis of the retinal gene expression after hypoxic preconditioning identifies candidate genes for neuroprotection

Project description:Neuroprotective therapies for retinal degeneration may be used to rescue retinal cells and preserve vision. Hypoxic preconditioning stabilizes the transcription factor HIF-1α in the retina and strongly protects photoreceptors in an animal model of light-induced retinal degeneration. Our data suggest that neuroprotection after hypoxic preconditioning of the retina is the result of the differential expression of a multitude of genes which may act in concert to protect visual cells against a toxic insult. Keywords: hypoxic preconditioning, neuroprotection, transcription-profile, retina

2009-01-10 | GSE14366 | GEO

A human tissue screen identifies a regulator of ER secretion as a brain size determinant [scRNA-Seq]

Project description:A human tissue screen identifies a regulator of ER secretion as a brain size determinant [scRNA-Seq]

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A human tissue screen identifies a regulator of ER secretion as a brain size determinant [RNA-Seq]

Project description:A human tissue screen identifies a regulator of ER secretion as a brain size determinant [RNA-Seq]

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Project description:Isolated perfused rat hearts exposed to isoflurane or ischemic preconditioning, a subgroup followed by 40 minutes of ischemia and 3 hours of reperfusion. Exact protocol available from the authors. Keywords = preconditioning Keywords = anesthetics Keywords = ischemia/reperfusion Keywords: other

2004-07-30 | GSE1616 | GEO

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