NIA CARD Coriell Cell Lines
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ABSTRACT:
PROVIDER: PRJNA943331 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Whole-genome sequencing of 32 Coriell human cell lines
| PRJEB87628 | ENA
Project description:Transcriptional profiling of Mycobacterium smegmatis comparing the effects of wild type levels of CarD expression with CarD depletion. Transcriptional profile of the mgm1701 control strain was compared with that of mgm1703, a strain where CarD transcription is regulated by a Tet-On system. Both strains were grown in the absence of anhydrotetracline for 13 hours in nutrient rich broth to allow for depletion of CarD transcription in mgm1703.
2009-07-01 | GSE15437 | GEO
Project description:Human fibroblast of different age were investigated for their gene expression profiles in quiescence. Cultures were established from skin samples derived from young and old donors, obtained from the NIA Aging Cell Repository (Coriell Institute for Medical Research, Camden, NJ). All cell lines originated from 2 mm punch biopsies taken from the medial aspect of the upper arm. The donors were members of the Baltimore Longitudinal Study of Aging (BLSA) where they were characterized as "healthy" indviduals. The cell lines investigated had normal karyotypes.
2010-02-26 | GSE15829 | GEO
Project description:Transcriptional profiling of Mycobacterium smegmatis comparing the effects of wild type levels of CarD expression with CarD depletion. Transcriptional profile of the mgm1701 control strain was compared with that of mgm1703, a strain where CarD transcription is regulated by a Tet-On system. Both strains were grown in the absence of anhydrotetracline for 13 hours in nutrient rich broth to allow for depletion of CarD transcription in mgm1703. Two condition experiment, control (mgm1701) vs CarD depletion (mgm1703). 3 biological replicates of each strain.
2010-05-16 | E-GEOD-15437 | biostudies-arrayexpress
Project description:NIA/NIH AGEMAP Multiple Tissues
| PRJNA103897 | ENA
Project description:Genome-wide transcriptome analysis of expression changes in EBV transformed cell lines from the Coriell Cell Repository in Parkinson and Control subjects.
2010-02-04 | GSE20153 | GEO
Project description:Bacterial transcription is a tightly regulated process critical for gene expression, adaptation and cell survival. CarD is an essential transcription factor in mycobacteria involved in regulation of gene expression. Here, we searched for CarD interaction partners in the model organism Mycobacterium smegmatis and identified two candidate proteins: ApeB (MSMEG_5828) and an uncharacterized protein, which we named CrsL (MSMEG_5890). While ApeB interacted with CarD only when CarD was overexpressed, CrsL associated with CarD at its physiological levels. We therefore focused on CrsL. It is a small protein of 5.7 kDa that we show by NMR to be intrinsically disordered. Genes encoding CrsL homologs are present in Actinobacteria including pathogenic species such as Mycobacterium tuberculosis. We demonstrate that CrsL directly interacts with CarD and both proteins bind to RNAP. Moreover, we show by ChIP-seq that CrsL associates with promoter regions of actively transcribed genes and ~75 % of these regions are associated also with CarD. By RNA-seq experiments with crsL and carD knockdown strains, we reveal ~50 % and ~66 % overlap between differentially expressed genes in the two strains in exponential and stationary phase, respectively. CrsL represses the expression of DesA desaturase and RNA helicase MSMEG_1930 gene, which are important for adaptation to cold stress. Furthermore, CrsL promotes the growth of M. smegmatis at elevated temperature. In summary, this study identifies CrsL as a novel actinobacterial transcription factor and provides a basis for its further investigation.
2025-12-22 | PXD058166 | Pride