Aspergillus nidulans
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ABSTRACT:
PROVIDER: PRJNA94419 | ENA |
REPOSITORIES: ENA
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Project description:Nitrogen and carbon repression general regulatory systems modulate the expression of target genes participating in utilization of alternative nitrogen/carbon sources, resulting in transcription only when preferred sources are limiting. In Aspergillus nidulans, the GATA transcription factor AreB was identified as nitrogen regulator whose activity depends on both nitrogen and carbon source (Conlon et al., 2001; Macios et al., 2012; Chudzicka - Ormanec et al., 2019). In this work, we performed a transcriptomic analysis of areB deletion and the wild type strains grown under different carbon/nitrogen conditions and showed that AreB participates both in nitrogen and carbon regulation. Observed positive and negative effects of areB deletion are at least partially, indirect as the expression of some transcription factor coding genes is changed in areB deletion strain.
2025-12-31 | GSE245665 | GEO
Project description:Transcriptional profiling of Aspergillus nidulans comparing control (growing on glucose) and carbon starving cultures.
2020-04-08 | GSE42610 | GEO
Project description:In Aspergillus nidulans, nitrogen and carbon metabolism are under the control of wide-domain regulatory systems, including nitrogen metabolite repression, carbon catabolite repression. Transcriptomic analysis of the wild type strain grown under different combinations of carbon and nitrogen regimes was performed, to identify differentially regulated genes. Carbon metabolism predominates as the most important regulatory signal but for many genes, both carbon and nitrogen metabolisms coordinate regulation.
2019-05-15 | GSE115021 | GEO
Project description:Transcriptome of A. nidulans ∆pkaA strain when grown on complete media (CM) and transferred to minimal media plus avicel as a sole carbon source for 8 and 24 hours
2015-07-15 | GSE70917 | GEO
Project description:Stranded RNA-seq of wild type Aspergillus nidulans during growth on a range of nitrogn sources
2021-05-06 | GSE145035 | GEO
Project description:Aspergillus nidulans is a model organism for aspergilli, an important group of filamentous fungi that encompasses human and plant pathogens, as well as industrial cell factories. Aspergilli have a highly diversified metabolism and both in connection with their biotechnological application as well as their interaction with other cells (humans or plants), it is valuable to understand how their metabolism is regulated. We therefore performed genome-wide transcription analysis of A. nidulans grown on three different carbon sources (glucose, glycerol, and ethanol) with the objective to identify global regulatory structures. We furthermore reconstructed the complete metabolic network of this organism, and this resulted in linking of 666 genes with metabolic functions, as well as assigning metabolic roles to 472 genes that had not been annotated earlier. Through combinations of the reconstructed metabolic network and the transcription data, we identified subnetwork structures that pointed to coordinated regulation of genes involved in many different parts of the metabolism. Keywords: carbon sources, metabolism, comparative genomics
2006-05-30 | GSE4578 | GEO
Project description:Transcriptome of A. nidulans TNO2a3, ∆snfA and ∆schA strains when grown on complete media (CM) and transferred to minimal media plus avicel as a sole carbon source for 8 and 24 hours.
2013-07-16 | GSE47810 | GEO
Project description:Transcriptome of A. nidulans R21 and ∆gprH strains when grown on MM+1% glucose for 24 hours and transferred to MM with no carbon for 4 and 8 hours
2015-08-01 | GSE65282 | GEO
Project description:Although tyrosol is a quorum-sensing molecule of Candida species, it has antifungal activity at supraphysiological concentrations. Here, we studied the effect of tyrosol on the physiology and genome-wide transcription of Aspergillus nidulans to gain insight into the background of the antifungal activity of this compound. Tyrosol efficiently reduced germination of conidia and the growth on various carbon sources at a concentration of 35 mM. The growth inhibition was fungistatic rather than fungicide on glucose and was accompanied with downregulation of 2199 genes related to e.g. mitotic cell cycle, glycolysis, nitrate and sulphate assimilation, chitin biosynthesis, and upregulation of 2250 genes involved in e.g. lipid catabolism, amino acid degradation and lactose utilization. Tyrosol treatment also upregulated genes encoding glutathione-S-transferases (GSTs), increased specific GST activities and the glutathione (GSH) content of the cells, suggesting that A. nidulans can detoxify tyrosol in a GSH-dependent manner even though this process was weak. Tyrosol did not induce oxidative stress in this species, but upregulated “response to nutrient levels”, “regulation of nitrogen utilization”, “carbon catabolite activation of transcription” and “autophagy” genes. Tyrosol may have disturbed the regulation and orchestration of cellular metabolism, leading to impaired use of nutrients, which resulted in growth reduction.
2023-08-22 | GSE223010 | GEO
Project description:Using genetic engineering tools available for the model organism Aspergillus nidulans, we constructed two recombinant strains; one expressing the model polyketide Penicillium griseofulvum 6-methylsalicylic acid (6-MSA) polyketide synthase gene, and one expressing the 6-MSA gene and overexpressing the native phosphoketolase (phk) for increasing the pool of polyketide precursor levels. The physiology of the recombinant strains and a reference wild type were characterized on glucose, xylose, glycerol and ethanol medium in controlled bioreactors. Glucose was found to be the preferable carbon source for 6-MSA production and 6-MSA titers up to 455 mg/L were achieved. Our findings indicate that overexpression of phk does not directly improve 6-MSA production on glucose but if the lower glycolysis is lowered, it is possible to obtain quite high conversion yields of sugar to 6-MSA. Systems biology tools were employed for in-depth analysis of the metabolic processes. Transcriptome analysis of 6-MSA producing strains on glucose and xylose in the presence and absence of phk overexpression combined with flux and physiology data enabled us to propose a model of phk/6msas interaction describing two different responses influencing 6-MSA production. Four strains on two carbon sources
2009-02-27 | E-GEOD-12859 | biostudies-arrayexpress