Aspergillus nidulans
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ABSTRACT:
PROVIDER: PRJNA94419 | ENA |
REPOSITORIES: ENA
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Project description:Nitrogen and carbon repression general regulatory systems modulate the expression of target genes participating in utilization of alternative nitrogen/carbon sources, resulting in transcription only when preferred sources are limiting. In Aspergillus nidulans, the GATA transcription factor AreB was identified as nitrogen regulator whose activity depends on both nitrogen and carbon source (Conlon et al., 2001; Macios et al., 2012; Chudzicka - Ormanec et al., 2019). In this work, we performed a transcriptomic analysis of areB deletion and the wild type strains grown under different carbon/nitrogen conditions and showed that AreB participates both in nitrogen and carbon regulation. Observed positive and negative effects of areB deletion are at least partially, indirect as the expression of some transcription factor coding genes is changed in areB deletion strain.
2025-12-31 | GSE245665 | GEO
Project description:Transcriptional profiling of Aspergillus nidulans comparing control (growing on glucose) and carbon starving cultures.
2020-04-08 | GSE42610 | GEO
Project description:In Aspergillus nidulans, nitrogen and carbon metabolism are under the control of wide-domain regulatory systems, including nitrogen metabolite repression, carbon catabolite repression. Transcriptomic analysis of the wild type strain grown under different combinations of carbon and nitrogen regimes was performed, to identify differentially regulated genes. Carbon metabolism predominates as the most important regulatory signal but for many genes, both carbon and nitrogen metabolisms coordinate regulation.
2019-05-15 | GSE115021 | GEO
Project description:Transcriptome of A. nidulans ∆pkaA strain when grown on complete media (CM) and transferred to minimal media plus avicel as a sole carbon source for 8 and 24 hours
2015-07-15 | GSE70917 | GEO
Project description:Stranded RNA-seq of wild type Aspergillus nidulans during growth on a range of nitrogn sources
2021-05-06 | GSE145035 | GEO
Project description:Aspergillus nidulans is a model organism for aspergilli, an important group of filamentous fungi that encompasses human and plant pathogens, as well as industrial cell factories. Aspergilli have a highly diversified metabolism and both in connection with their biotechnological application as well as their interaction with other cells (humans or plants), it is valuable to understand how their metabolism is regulated. We therefore performed genome-wide transcription analysis of A. nidulans grown on three different carbon sources (glucose, glycerol, and ethanol) with the objective to identify global regulatory structures. We furthermore reconstructed the complete metabolic network of this organism, and this resulted in linking of 666 genes with metabolic functions, as well as assigning metabolic roles to 472 genes that had not been annotated earlier. Through combinations of the reconstructed metabolic network and the transcription data, we identified subnetwork structures that pointed to coordinated regulation of genes involved in many different parts of the metabolism. Keywords: carbon sources, metabolism, comparative genomics
2006-05-30 | GSE4578 | GEO
Project description:Transcriptome of A. nidulans TNO2a3, ∆snfA and ∆schA strains when grown on complete media (CM) and transferred to minimal media plus avicel as a sole carbon source for 8 and 24 hours.
2013-07-16 | GSE47810 | GEO
Project description:Transcriptome of A. nidulans R21 and ∆gprH strains when grown on MM+1% glucose for 24 hours and transferred to MM with no carbon for 4 and 8 hours
2015-08-01 | GSE65282 | GEO
Project description:Although tyrosol is a quorum-sensing molecule of Candida species, it has antifungal activity at supraphysiological concentrations. Here, we studied the effect of tyrosol on the physiology and genome-wide transcription of Aspergillus nidulans to gain insight into the background of the antifungal activity of this compound. Tyrosol efficiently reduced germination of conidia and the growth on various carbon sources at a concentration of 35 mM. The growth inhibition was fungistatic rather than fungicide on glucose and was accompanied with downregulation of 2199 genes related to e.g. mitotic cell cycle, glycolysis, nitrate and sulphate assimilation, chitin biosynthesis, and upregulation of 2250 genes involved in e.g. lipid catabolism, amino acid degradation and lactose utilization. Tyrosol treatment also upregulated genes encoding glutathione-S-transferases (GSTs), increased specific GST activities and the glutathione (GSH) content of the cells, suggesting that A. nidulans can detoxify tyrosol in a GSH-dependent manner even though this process was weak. Tyrosol did not induce oxidative stress in this species, but upregulated “response to nutrient levels”, “regulation of nitrogen utilization”, “carbon catabolite activation of transcription” and “autophagy” genes. Tyrosol may have disturbed the regulation and orchestration of cellular metabolism, leading to impaired use of nutrients, which resulted in growth reduction.
2023-08-22 | GSE223010 | GEO
Project description:Glutathione (GSH) is an abundant and widely distributed antioxidant in fungi. Hence, understanding cellular GSH metabolism is of vital importance to deciphering redox regulation in these microorganisms. In this study, we generated dugB (AN1879), dugC (AN1092), and dugB dugC double deletion mutants which display disruption of the GSH degradation pathway in Aspergillus nidulans. Deletion of dugB, dugC or both resulted in a moderate increase in GSH content under growing conditions and substantially slowed down the depletion of GSH pools under carbon starvation. Inactivation of dug genes caused reduced accumulation of reactive oxygen species, decreased autolytic cell wall degradation and extracellular enzyme production, increased sterigmatocystin formation but decreased viability in starving cultures. Changes in the transcriptomes suggested that enzyme secretions were controlled at post transcriptional level. In contrast, secondary metabolite production was also regulated at the level of mRNA abundance. Based on these findings, we suggest that GSH connects starvation and redox regulation to each other: A. nidulans cells utilize GSH as stored carbon source during starvation. The reduction of GSH contents of cells alters the redox state activating regulatory pathways responsible for carbon starvation stress responses. Under glucose rich conditions, inactivation of dug genes reduced conidia production of surface cultures, disturbed sexual development and down-regulated the transcription of genes encoding MAP kinase pathway proteins (e.g. steC, sskB, pbsA, hogA, mkkA) or proteins involved in the regulation of conidiogenesis or sexual differentiation (e.g. flbA,C,E, nosA, rosA, nsdC,D). These finding indicates that the authority of redox regulation goes far beyond the protection against redox stress; it affects development, stress responses (other than redox stress) and secondary metabolism as well.
2021-03-02 | GSE151461 | GEO