Project description:Transgenic wheat plants of cv. Bobwhite were generated that constitutively overexpress class III peroxidase cDNA TaPrx103 (previously TaPERO) under the control of the epidermis-specific GstA1 promoter. Several transgenic lines show enhanced pathogen resistance. In order to test the concept of substantial equivalence, wildtype and transgenic lines were subjected to pathogen attack by Blumeria graminis f.sp. tritici, followed by transcript profiling using the barleyPGRC1 cDNA array.
Project description:Time course: Interaction of Blumeria graminis f. sp. hordei with Hordeum vulgare, Ingrid (leaf) and Blumeria graminis f. sp. tritici with Hordeum vulgare, Ingrid (leaf)
Project description:Time course: Interaction of Blumeria graminis f. sp. hordei with Hordeum vulgare, Ingrid (leaf epidermis) and Blumeria graminis f. sp. tritici with Hordeum vulgare, Ingrid (leaf epidermis)
Project description:To analyze RACB- and Bgh (Blumeria graminis f.sp. hordei)-dependently expressed genes we used wild type and transgenic barley misexpressing the susceptibility factor RACB
Project description:Puccinia graminis f.sp. tritici (Pgt), the causal agent of stem rust disease in wheat, is one of the most destructive pathogens and can cause severe yield losses. Here, we utilize Hi-C sequencing technology to scaffold and phase the haplotypes for the genome assembly of a US Pgt isolate 99KS76A-1.
Project description:Large scale proteomics of Blumeria graminis f.sp. hordei DH14 has been conducted with a new ORF database containing candidate secreted effector proteins (CSEPs). With a comparative approach, CSEPs only deteted in Haustoria (the interacting cells) containing tissue were identified.
2012-12-06 | PRD000730 | Pride
Project description:Genome assembly of Blumeria graminis f.sp. tritici isolate CHVD_042201
Project description:The assess the effect of AvrSr35 knock-out on host-pathogen interaction in the compatible host, we have performed time-course analysis of leaf transcriptomes obtained by infecting susceptible wheat cultivar Fielder with the wild type Puccinia graminis f.sp. tritici (Pgt) isolate 99KS76A-1 and its three mutants, M1, M4 and M7, that carry loss-of-function mutations in the AvrSr35 gene.
Project description:Powdery mildew (PM) is one of the most important and widespread plant diseases caused by obligate biotrophic Ascomycete fungi in the order of Erysiphales. Monocot PM fungi such as Blumeria graminis f.sp. hordei (Bgh) infectious on barley and B. graminis f.sp. tritici (Bgt) infectious on wheat exhibit high-level of host-specialization. By contrast, many dicot PM fungi display rather broad host ranges. To understand why different PM fungi adopt distinct modes of host-adaption, we sequenced the genomes of four dicot PM strains belonging to Golovinomyces cichoracearum (GcC1, GcM1, GcM3) or Oidium neolycopersici (OnM2) and conducted comparative sequence analyses. PM fungi have highly repetitive genomes that are difficult to perform gene prediction. By combing RNA-seq expression evidence with ab initio gene prediction, we successfully improved the number of predicted genes from 4000 to 6000. By comparing the transcriptional profiling from haustoria with mycelia in OnM2 and GcM3, we found that 86%-96% of the predicted genes are expressed in mycelia and/or haustoria, indicating an efficient expression system of PM fungi. Besides, our results showed that gene regulation mechanisms in haustorial cells maybe under gone a much higher level of diversification between OnM2 and GcM3, since they share only a small proportion (21%) of genes up-regulated in huastoria cells. Notably, a higher proportion of candidate effector genes are selectively up-regulated in haustorial cells, agreeing with their function in suppressing host defense and facilitating nutrient uptake.